柴胡总皂苷精制品大鼠肝毒性病理学研究

Experimental Study on the Pathology of Hepatotoxicity Induced by Saikosaponins in Rats

目的观察连续15d给予大鼠不同剂量的柴胡总皂苷醇洗脱精制样品导致大鼠肝毒性病理学损伤程度。方法分别给大鼠灌胃高、中、低剂量的柴胡总皂苷醇洗脱精制样品,按柴胡总皂苷计算,高、中、低剂量组分别为300,150,50mg.kg-1,除观察一般状况外,剖杀大鼠,精密称取心、肝、脾、肺、肾脏重量,计算脏体比值;进行肝脏病理组织学检查。结果不同剂量的柴胡总皂苷醇洗脱精制样品可导致大鼠体重下降,肝脏重量和肝体比值增大,病理学检查可见不同程度的肝组织损伤;上述变化随剂量的增加而逐渐加重,与空白组比较有明显差异。结论柴胡对大鼠肝毒性损伤程度与柴胡用药剂量、柴胡总皂苷含量呈剂量依赖关系。柴胡总皂苷是柴胡导致肝毒性的毒效部位,柴胡总皂苷醇洗脱精制品(81.9%)在一定剂量下,连续给药15d即可导致大鼠明显的肝脏器质性病变,其细胞损伤乃至坏死是主要病理改变。

Objective The hepatotoxicity induced was studied after given multiple administration of Saikosaponins to mice. Methods The rats were divided into different groups of high, medium and low dose. besides the normal conditions were observed, the heart, liver, spleen, lung, kidney weigh and volume ratio were calculated, the changes of liver morphology was observed after administration. Results The liver weight and volume ratio was increased, while the rat＇s weight were decreased. Pathological exanfination showed different degree of liver pathology tissue damage, and the damage of liver in rats show an obvious dosage relationship. Conclusion the damage of liver show a relationship with the dose of Bupleurum chinense. Saikosaponins is the toxicity site, and this show a organic disease of liver after given Saikosaponins 15 days, the damage and necrosis were the main pathological changes .