三氯杀螨醇对泥鳅血清GST和AChE活力的影响

Effects of Dicofol on Activities of GST and AChE in Blood Serum of Loach(Misgurnus anguillicaudatus)

生化指标能更灵敏地反映环境污染的状况和危害程度,日益成为环境领域研究的热点。为研究三氯杀螨醇对泥鳅生化指标的影响,将泥鳅暴露于含不同剂量三氯杀螨醇的人工沉积物中,在暴露24、48和96 h后采集泥鳅血液样本,测定血清中谷胱甘肽硫转移酶(GST)和乙酰胆碱酯酶(AChE)的活力。结果表明:泥鳅在人工沉积物中暴露24和96 h后,血清中GST活力随三氯杀螨醇剂量的升高呈先升高后降低的趋势,暴露48 h后GST活力随染毒剂量的升高而不断升高;血清中AChE活力随三氯杀螨醇剂量的升高逐渐下降;血清中GST和AChE活力均随染毒时间的延长而降低,有较显著的时间-效应关系。GST和AChE活力的变化能够灵敏地反映出三氯杀螨醇对泥鳅的毒性大小,初步推断可作为生物标志物来评价三氯杀螨醇对水生生物的生化毒性。

Biochemical indices are more sensitive to reflect the damage caused by pollutants in the environment,and thus have attracted increasing attention in the environment field.To study the effect of dicofol（DCF） on biochemical indices of loach（Misgurnus anguillicaudatus）,loaches were exposed in artificial sediments containing various dosages of DCF,and blood samples were collected respectively after 24,48 and 96 h exposure to determine the activities of glutathione S-transferase（GST） and acetylcholinesterase（AChE） in blood serum.Results showed that after 24 and 96 h exposure,the GST activities were induced by DCF at lower dosages and inhibited by DCF at higher dosages,while after 48 h exposure,the GST activities were induced by DCF at different dosages and the induction effect was enhanced with increasing the dosages.The AChE activities in blood serum decreased as the DCF dosages increased.Both GST and AChE activities tended to decrease with the extension of exposure time,demonstrating a fine time-effect relationship.It is indicated that the activities of GST and AChE in blood serum could obviously reflect the toxicity of DCF to loach,so GST and AChE were suggested preliminarily as biomarkers to assess the biochemical toxicity of DCF to aquatic organisms.