维药神香草乙酸乙酯部位对大鼠实验性哮喘炎症反应的影响

Effect of Uighur Medicine Hyssopus officinalis L. Ethly Aectate on Inflammatory Response in Asthma Rats

通过观察哮喘大鼠肺组织病理学改变,测定支气管肺泡灌洗液(BALF)中内皮素-1(ET-1)水平及细胞总数、淋巴细胞(Ly)、嗜酸性粒细胞(Eos)、中性粒细胞(Neu)的百分比,血清中的内皮素-1(ET-1)、白介素-2(IL-2)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)水平,探讨神香草乙酸乙酯部位对哮喘炎症反应的影响,阐明维药神香草抗哮喘的部分作用机制。将大鼠随机分为正常对照组,哮喘模型组,氨茶碱阳性对照组,神香草乙酸乙酯部位高、中、低剂量治疗组。采用卵清白蛋白(OVA)、氢氧化铝及百白破疫苗联合致敏和OVA生理盐水雾化激发的方法制备哮喘模型。测定BALF中细胞总数,Ly、Eos、Neu的百分比;采用酶联免疫吸附实验法(ELISA)检测血清及BALF中ET-1水平,放射免疫法检测血清中ET-1、IL-2、IL-6、TNF-α水平。结果显示,与模型组相比,各治疗组大鼠BALF中细胞总数,Ly、Eos,Neu的百分比,ET-1水平及血清中的ET-1、IL-2、IL-6、TNF-α水平均明显下降,差异有统计学意义(P<0.05);神香草乙酸乙酯部位各剂量组之间相互比较,BALF及血清中的以上指标差异均有统计学意义(P<0.05),呈剂量依赖性趋势。由此推测,维药神香草可能通过抑制IL-2、IL-6、ET-1、TNF-α、Ly、Eos、Neu的分泌,减轻哮喘的炎症反应。

The effect of Uighur medicine Hyssopus offwinalis L. ethly aectate on pathological changes in lung tissues of asthmatic rats was observed, and the level of ET-1, the total number of cells and the percentage of lymphocytes （Ly）, eosinophils （Eos）, and neutrophils （Neu） in BALF, the levels of ET-1, IL-1, IL-6, and TNF-α in serum were determined for the study of the mechanism of its treatment of asthma. The rats were randomly divided into the normal control group, the asthma model group, the aminophylline treatment group, and the Hyssopus ethyl acetate of high, medium and low dose treatment groups. They were sensitized with OVA, Al（OH）3 and DPT vaccine and were then challenged with inhalation of aerosolized OVA solution for preparation of the asthma model. The levels of serum and BALF ET-1 were determined with ELISA, and the total number of cells and the percentages of Ly, Eos, and Neu in BALF and the levels of serum IL-1, IL-6, and TNF-α were determined with radioimmunoassay. The results show that as compared with the asthma model group, the level of ET-1, the total number of cells and the percentage of Ly, Eos, and Neu in BALF, and^the levels of serum ET-1, IL-1, IL-6, and TNF-α are significantly lower in those of treated groups （P〈0.05）. In the comparisons among different dose treatment groups, the level of ET-1, the total number of cells and the percentage of Ly, Eos, and Neu in BALF and the levels of serum ET-1, IL-1, IL-6, and TNF-α are significantly different （P〈0.05） and it is shown that the dose-dependent trend to aggravate the inflammatory response ofasthma, Hyssopus officinalis L. may inhibit the secretion of ET-1, IL-2, IL-6, TNF-α, Ly, Eos, and Neu, reducing the inflammatory response of asthma.