流感检测阴性流感样病例手足口病毒检测结果分析

Analysis of the hand-foot-mouth disease virus with influenza virus nuclear acid negative samples of ILI patients

目的为了解流感样病例流感病毒核酸检测阴性样品中手足口病的感染情况,为HFMD正确的临床诊断提供科学依据。方法收集153份流感样病例流感检测阴性的样本,提取病毒RNA,采用RT-PCR一步法检测人肠道病毒(PE)的5‘-UTR区基因、肠道病毒71型(EV71)的VP3-VP1区基因和柯萨奇病毒A16型(CVA16)的VP3-VP1区基因,采用琼脂糖凝胶电泳法分析PCR产物。结果 153份流感样病例流感阴性的样本中,有24例样本PE阳性(15.69%),5份为EV71阳性(3.27%),19份其他肠道病毒阳性(12.42%),没有CVA16感染;EV71阳性病例的年龄为10个月～4岁的儿童。结论在153份流感样病例流感检测阴性样本中,检出24份PE阳性、5份EV71阳性感染。

Objective To reveal the status of HFMD in the negative sample of influenza,in the cause of correcting clinical diagnosis for HFMD.Methods The viral RNA were extracted from 153 cases of the negative samples of influenza,and the viral RNA were taken to detect the 5＇-UTR gene of PE,the VP3-VP1 genes of EV71 and CVA16 by RT-PCR assay.The PCR products were analyzed by agarose gen electrophoresis.Results Detection and analysis of the hand-foot-mouth disease with 153 cases of the negative samples of influenza were performed,24（15.69%） were PE positive,5（3.27%） were EV71 positive,19 were other EV positive（12.42%）and none of the sample was CVA16 positive.The age of patients who were EV71 positive was from 10 months to 4 years old.Conclusions 24 PE positive and 5 EV71 positive were found in the 153 cases of the negative samples of influenza.