HPLC法测定消渴降糖胶囊中槲皮素的含量

Determination of quercetin in Xiaoke Jiangtang Capsule by HPLC

目的:探讨用高效液相色谱法检测消渴降糖胶囊中槲皮素的含量。方法:应用高效液相色谱法,采用C18柱,以甲醇-0.4%磷酸(55∶45)为流动相,以360 nm为检测波长,检测消渴降糖胶囊中槲皮素的含量。结果:槲皮素峰与相邻色谱峰能有效分离,柱效较高,槲皮素对照品进样量在0.056～0.560μg范围内与峰面积呈良好的线性相关,回归方程为Y=40 103.2X-561.4,相关系数r值为0.999 8;精密度RSD=0.9%;10 h稳定性试验RSD=1.10%;重复性试验结果显示,5份样品含量的变异系数RSD=1.30%;3个不同浓度的加样回收试验,平均回收率为98.7%,RSD=0.90%。结论:采用高效液相色谱法测定消渴降糖胶囊中槲皮素的含量,专属性强,结果可靠,重现性好,可用于消渴降糖胶囊中槲皮素的含量测定。

Objective： To explore the method of high performance liquid chromatography （HPLC） in the determination of quercetin in Xiaoke Jiangtang Capsule. Methods： High performance liquid chromatography was employed to test the quereetin contents of Xiaoke Jiangtang Capsules. C18 column, mobile phase of methanol-0.4% phosphoric acid （55：45） and detection Wavelength of 360 nm were adopted. Results： The peak of quercetin and the adjacent peaks could be differentiated with high column efficiency. The quercetin reference standard sample size of 0.056 to 0.560 μg had a good linear correlation with the regression equation of Y=40 103.2X-561.4 and peak area （r=0.999 8）, the precision RSD was 0.9% and the RSD of 10 hours stability test result was 1.10%. In repeated tests, the RSD of coefficient of variation among the 5 samples content was 1.30%; added sample tests of 3 different concentrations had average recovery rate of 98.7% and RSD was 0.90%. Conclusion： It can be concluded that, HPLC is stable and reliable in the determination of quercetin in Xiaoke Jiangtang Capsule.