摘要
目的观察西洛他唑对人脐静脉内皮细胞(HUVECs)血管细胞黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)mRNA表达的影响,探讨西洛他唑可能的抗动脉粥样硬化作用机制。方法将HUVECs用不同浓度的西洛他唑(0μg/L、0.05μg/L、0.1μg/L、1.0μg/L、10μg/L)溶液处理1小时后,用肿瘤坏死因子α(TNF-α)10μg/L诱导24小时。半定量复合逆转录聚合酶链反应(RT-PCR)测定黏附分子VCAM-1和ICAM-1mRNA的表达。结果 TNF-α能上调VCAM-1和ICAM-1的表达,西洛他唑在一定程度上可抑制上述作用,随着西洛他唑浓度的增加,ICAM-1mRNA表达水平逐步下降,分别为0.239±0.012、0.205±0.012、0.166±0.010、0.136±0.008,VCAM-1mRNA表达水平也逐步下降,分别为0.114±0.048、0.093±0.051、0.083±0.045、0.068±0.039。结论西洛他唑可抑制TNF-α诱导的HUVECs的黏附分子VCAM-1和ICAM-1mRNA表达,提示西洛他唑的抗动脉粥样硬化作用可能是通过阻止血单核细胞向血管内皮细胞聚集和黏附实现的。
Objective To investigate the effect of cilostazol on vascular adhesion molecule-1（VCAM-1） and intercellular adhesion molecule-1（ICAM-1） expression in human umbilical vein endothelial cells（HUVECs） and the possible mechanism of the antiatherosclerotic effect. Methods HUVECs were cultivated in vitro,pretreated with cilostazol in different concentrations（0 μg/L,0.05 μg/L,0.1 μg/L,1.0 μg/L,10 μg/L） for one hour,and by stimulation with tumor necrosis facter-alpha（TNF-α）（10 μg/L） for 24 hours.Reverse transcription-polymerase chain reaction（RT-PCR） was performed to detect the expression of VCAM-1 and ICAM-1 on mRNA. Results Expressions of VCAM-1 and ICAM-1 on mRNA which were up-regulated by TNF-α were significantly suppressed by cilostazol in a dose dependent manner,with the increase of cilostazol concentration,expression of ICAM-1 on mRNA dropped gradually,0.239±0.012,0.205±0.012,0.166±0.010 and 0.136±0.008,expressions of VCAM-1 on mRNA also reduced,0.114±0.048,0.093±0.051,0.083±0.045 and 0.068±0.039. Conclusion The inhibition of function of cilostazol in VCAM-1 and ICAM-1 expression induced by TNF-α,demonstrates that cilostazol has the effect of inhibiting the adhesion of blood mononuclear cell to HUVECs,and this contributes to antiatherosclerotic effect of cilostazol on the onset and development of atherosclerosis.
出处
《临床荟萃》
CAS
2011年第21期1868-1870,1873,共4页
Clinical Focus
