强保留固定相的色谱特性

Chromatographic Characterization of a Highly Retentive Stationary Phase

在新方法的发展过程当中,色谱方法最重要的目标之一是得到具有一致性且可重现的分离。选取具有高重现性和良好性能的高效液相色谱(HPLC)柱是实现这一目标最基本的要素。对装入HPLC柱的色谱介质进行物理测试毋庸置疑也是非常重要的,该测试可以揭示HPLC柱的部分色谱特性。柱的本身特性以及如何与一个宽范围的分析物相互作用只能通过严格的色谱评估来获得。

When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. The selection of a highly reproducible and well--characterized HPLC column is essential if this goal is to be attained. The physical testing of the chromatographic media that is packed inside an HPLC column, while undoubtedly important, will reveal only some of the characteristics of the column. The true nature of the column and how it will interact with a wide range of analytes can only be understood through rigorous chromatographic evaluation.