应用荧光标记PCR对散发性结直肠癌进行微卫星不稳定性检测

Detection of Microsatellite Instability in Sporadic Colorectal Cancer Using Fluorescence-labelled Primers PCR

目的检测散发性结直肠癌微卫星情况并探讨其临床意义。方法对41例散发性结直肠癌患者,应用荧光标记PCR检测国际通用的5个微卫星位点,分析微卫星不稳定性(MSI)与临床病理参数的关系。结果 MSI检出率为24.39%(10/41),其中MSI-H为9.76%(4/41),荧光标记PCR与多重荧光PCR检出率接近;散发性结直肠癌MSI与TNM分期、性别、年龄、肿瘤部位、CEA状态无关(P>0.05)。结论荧光标记PCR是检测MSI有效快速的方法之一;散发性结直肠癌MSI与临床病理因素无关,但与预后的关系还不明确。

Objective To analyze the relationship between microsatellite instability and clinicopathological features in sporadic colorectal cancer and its clinical significance. Methods Fluorescence-labelled primers PCR were used to detect the microsatellite instability with global five microsatellite loci in 41 patients with sporadic colorectal cancer. The clinicopathological features characterized by microsatellite instability and microsatellite stability were compared statistically. Results The microsatellite instability was 24.39% in sporadic colorectal cancer patients, including 4 cases（9.76% ）with MSI-high, it was similar about detection rate between the fluorescence-labelled primers PCR and fluorescence-labelled primers multiplex PCR. The clinicopathological variables, such as TNM staging, gender, age, tumor position and CEA condition between MSI-and MSS-positive colorectal cancer were not statistically different （P 〉 0.05）. Conclusion The fluorescence-labelled primers PCR is one of the good methods for MSI. The sporadic colorectal cancer MSI has no relationship with its clinicopathological features, but it is not clear in the relationship between MSI and prognosis.