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瘦素抑制肝星状细胞中SREBP-1c的表达 被引量：2

Leptin inhibits SREBP-1c expression in hepatic stellate cells
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摘要 目的:揭示瘦素是否调控培养的肝星状细胞中固醇调节元件结合蛋白-1c(SREBP-1c)的表达。方法:用瘦素孵育培养的肝星状细胞,分别通过Western Blot和real-time PCR检测SREBP-1c的蛋白及其mRNA水平,并通过双荧光素酶报告基因检测SREBP-1c启动子活性。结果:瘦素明显抑制SREBP-1c的蛋白表达,mRNA的转录,瘦素还能抑制SREBP-1c基因的启动子水平。结论:揭示了瘦素在肝纤维化发生中具有独特促进作用。 Objective： The aim of this study is to examine whether leptin regulates SREBP-1c gene expression in HSCs in vitro.Methods： Cultured HSCs was incubated with 100 ng/mL of leptin.The levels of SREBP-1c protein and mRNA were examined by Western Blot and real-time PCR,respesctively.The SREBP-1c promoter activity was analysed by transfection assay.Results： The results of the present study indicated that leptin significantly down-regulated the levels of SREBP-1c protein and mRNA.Furthermore,leptin also inhibited SREBP-1c promoter activity in HSCs.Conclusion： These results might provide new insights into the mechanisms of the unique role of leptin in the development of liver fibrosis.
作者 贾辛 翟旭光 牛明辉 周亚军
机构地区 南通大学医学院生化与分子生物学系
出处 《南通大学学报（医学版）》 2011年第5期318-320,共3页 Journal of Nantong University(Medical sciences)
基金 国家自然科学基金资助项目(30971117)
关键词 肝星状细胞 肝纤维化 瘦素 固醇调节元件结合蛋白-1C hepatic stellate cells liver fibrosis leptin sterol regulatory element binding protein-1c
分类号 R575.2 [医药卫生—消化系统]
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参考文献18

  • 1William WN Jr, Ceddia RB, Curl R. Leptin controls the fate of fatty acids in isolated rat white adipocytes[J]. J Endocrinol, 2002, 175(3):735-744.
  • 2Zhou YT, Wang ZW, Higa M, et al. Reversing adipocyte differentiation: implications for treatment of obesity[J]. Proc Natl Acad Sci U S A, 1999, 96(5):2391-2395.
  • 3Saxena NK, Ikeda K, Rockey DC, et al. Leptin in hepatic fibrosis: evidence for increased collagen production in stel- late ceils and lean littermates of ob/ob mice[J]. Hepatology, 2002, 35(4):762-771.
  • 4Tang Y, Chen A. Curcumin protects hepatic stellate cells a-gainst leptin-induced activation in vitro by accumulating intracellular lipids[J]. Endocrinology, 2010,. 151 (9):4168-4177.
  • 5Zhou Y, Jia X, Qin J, et al. Leptin inhibits PPARgamma gene expression in hepatic stellate ceils in the mouse model of liver damage[J]. Mol Cell Endocrinol, 2010, 323(2): 193-200.
  • 6Tsukamoto H. Adipogenic phenotype of hepatic stellate cells[J]. Alcohol Clin Exp Res, 2005, 29(11 Suppl):132S-133S.
  • 7Rangwala SM, Lazar MA. Transcriptional control of adipogenesis[J]. Annu Rev Nutr, 2000, 20:535-559.
  • 8She H, Xiong S, Hazra S, et al. Adipogenic transcriptional regulation of hepatic stellate cells[J]. J Biol Chem, 2005, 280(6):4959-4967.
  • 9Potter J J, Womack L, Mezey E, et al. Transdifferentiation of rat hepatic stellate cells results in leptin expression[J]. Biochem Biophys Res Commun, 1998, 244(1):178-182.
  • 10Zh0u Y, Jia X, Wang G, et at. PI-3K/AKT and ERK signaling pathways mediate leptin-induced inhibition of PPARgamma gene expression in primary rat hepatic stellate cells[J]. Mol Cell Biochem, 2009, 325(1-2):131-139.

二级参考文献12

  • 1张冬梅,张桂英,王涛,钟惠菊,陈文科.改善胰岛素抵抗药物治疗大鼠非酒精性脂肪肝病的实验研究[J].中华医学杂志,2006,86(18):1279-1283. 被引量：14
  • 2Tsochatzis E,Papatheodoridis GV,Manesis EK,et al.Metabolic syndrome is associated with severe fibrosis in chronic viral hepatitis and non-alcoholic steatohepatitis[J].Aliment Pharmacol Ther,2008,27(1):80-89.
  • 3Tsochatzis EA,Manolakopoulos S,Papatheodoridis GV,et al.Insulin resistance and metabolic syndrome in chronic liver diseases:old entities with new implications[J].Scand J Gastroenterol,2009,44(1):6-14.
  • 4Franzese A,Vajro P,Argenziano A,et al.Liver involvement in obese children.Ultrasonography and liver enzyme levels at diagnosis and during follow-up in an Italian population[J].Dig Dis Sci,1997,42(7):1428-1432.
  • 5Ekstedt M,Franzén LE,Mathiesen UL,et al.Long-term follow-up of patients with NAFLD and elevated liver enzymes[J].Hepatology,2006,44(4):865-873.
  • 6Tu H,Kastin AJ,Hsuchou H,et al.Soluble receptor inhibits leptin transport[J].J Cell Physiol,2008,214(2):301-305.
  • 7Laclaustra M,Corella D,Ordovas JM.Metabolic syndrome pathophysiology:the role of adipose tissue[J].Nutr Metab Cardiovasc Dis,2007,17(2):125-139.
  • 8Fischer-Posovszky P,Wabitsch M,Hochberg Z.Endocrinology of adipose tissue-an update[J].Horm Metab Res,2007,39(5):314-321.
  • 9Tsochatzis E,Papatheodoridis GV,Archimandritis AJ.The evolving role of leptin and adiponectin in chronic liver diseases[J].Am J Gastroenterol,2006,101(11):2629-2640.
  • 10Chan JL,Matarese G,Shetty GK,et al.Differential regulation of metabolic,neuroendocrine,and immune function by leptin in humans[J].Proc Natl Acad Sci USA,2006,103(22):8481-8486.

同被引文献22

  • 1姚晓敏,宋保亮,王灿华,张文静,林志新,李伯良.胆固醇平衡调控中的SREBP途径[J].上海交通大学学报（农业科学版）,2006,24(3):311-320. 被引量：13
  • 2邹颖,王勇,吴效科.过氧化物酶体增殖物活化受体与胰岛素抵抗[J].中国公共卫生,2007,23(3):371-372. 被引量：3
  • 3郭晓强,郭振清.SREBP介导的胆固醇生物合成反馈调节[J].生命的化学,2007,27(4):292-294. 被引量：7
  • 4Goldstein J L, Rawson R B, Brown M S. Mutant mammalian cells as tools to delineate the sterol regulatory element binding protein pathway for feedback regulation of lipid synthesis [J]. Arch Biochem Biophys, 2002, 397: 139-148.
  • 5Bennett M K, NgoT T, Athanikar J N, et al. Co-stimulation of promoter for low density lipoprotein receptor gene by sterol regu- latory element-binding protein and Spl is specifically disrupted by the yin yang 1 protein [J]. J Biol Chem, 1999, 274(19): 13025-13032.
  • 6Deng X, Cagen L M, Wilcox H G, et al. Regulation of the rat SREBP-lc promoter in primary rat hepatocytes [J]. Biochem Bioph Res Co, 2002, 290: 256-622.
  • 7Hua X, Yokoyama C, Wu J, et ol. SREBP-2, a second basic- helix-loop-helix-leucine zipper protein that stimulates tran- scription by binding to a sterol regulatory element [J]. Proc Natl Acad Sci USA, 1993 , 90(24): 11603-11607.
  • 8Ferre P, Foufelle F. SREBP-lc transcription factor and lipid homeostasis: clinical perspective[J]. Horm Res, 2007, 68 (2): 72-82.
  • 9Eber1e D, Hegarty B, Bossard P, et al. SREBP transcription fac- tors: master regulators of lipid homeostasis[J]. Biochimie, 2004, 86: 839- 848.
  • 10Vittoria I, Vito I, Francesco De S, et al. Transcription of the mitochondrial citrate carrier gene: Role of SREBP-1, upregula- tion by insulin and downregulation by PUFA[J]. Biochem Bioph Res Co, 2007, 356: 249-254.

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二级引证文献14

南通大学学报（医学版）
2011年 第5期

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