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DEC205在幽门螺杆菌感染胃上皮细胞中的表达

Expression of DEC205 in Helicobacter pylori-Infected Human Gastric Epithelial Cells
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摘要 目的:探讨幽门螺杆菌及其热休克蛋白60(H.pylori-HSP60)感染与胃上皮细胞表面DEC205受体的关系。方法:分别用H.pylori、H.pylori-HSP60及E.coli LPS刺激胃上皮细胞KATOⅢ,利用免疫荧光染色技术观察KATOⅢ细胞表面DEC205蛋白的表达变化,再利用RT-PCR技术,观察细胞中DEC205mRNA对上述抗原刺激后的变化。结果:H.pylori、H.pylori-HSP60及E.coli LPS的刺激明显引起细胞表面DEC205蛋白的表达以及细胞内DEC205 mRNA的产生。结论:H.pylori感染与胃上皮细胞表面的胞吞受体DEC205有着密切的关系。 Objective： To investigate the relationship between Helicobacer pylori and its heat shock protein 60 （H. pylori-HSP60） infection and human gastric epithelial cells of DEC205. Methods： After human gastric epithelial cells （KATOIII cells line） were infected with H. pylori, H. pylori-HSP60 and E.coli LPS. The expression of DEC205 protein on the surface of KATOIII cells was observed by im- munofluorescence staining. The expression of DEC205 mRNA in KATOIII cells was observed by RT-PCR. Results： The expression of DEC205 protein and mRNA were markedly increased following H. pylori, H. pylori-HSP60 and E.coli LPS stimulation in KATOIII cells. Conclusion： H. pylori infection may be closely related with the expression of DEC205 in human gastric epithelial cells.
作者 魏晓晴 吕广艳 金海威 崔颖 赵莹
机构地区 辽宁省医学细胞分子生物学重点实验室 大连医科大学口腔医学院基础教研室
出处 《现代生物医学进展》 CAS 2011年第21期4033-4035,共3页 Progress in Modern Biomedicine
基金 辽宁省教育厅2009年度高等学校科研项目计划资助项目(2009A195)
关键词 H.PYLORI DEC205 胃上皮细胞 H. pylori-HSP60 DEC205 Gastric epithelial cells
分类号 R378.2 [医药卫生—病原生物学]
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参考文献16

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二级参考文献18

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现代生物医学进展
2011年 第21期

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