黏蛋白4和酪氨酸激酶受体在非小细胞肺癌中的表达及意义

Expression and clinical significance of mucin 4 and tyrosine kinase receptor in non-small cell lung cancer

目的研究上皮中黏蛋白4(MUC4)和酪氨酸激酶受体(ErbB2)蛋白在非小细胞肺癌(NSCLC)中的表达、临床意义及两者的相关性。方法用免疫组化链霉菌抗生物素蛋白过氧化物酶连结(SP)法检测70例NSCLC(NSCLC组),30例癌旁组织(对照组)标本中MUC4和ErbB2基因蛋白的表达。结果 NSCLC中MUC4和ErbB2高表达率分别为22.9%(16/70)、47.1%(33/70)较癌旁组织均明显升高(P<0.05);MUC4和ErbB2均随NSCLC病理分化程度的降低,高表达率逐渐增高(P<0.05);NSCLC中MUC4和ErbB2临床Ⅲ～Ⅳ期的高表达率高于Ⅰ～Ⅱ期,且MUC4的表达差异有统计学意义;MUC4和ErbB2在伴淋巴结转移的37例NSCLC患者中的高表达率均较无淋巴结转移者明显升高(P<0.05)。结论 MUC4表达与NSCLC患者的病理分级、临床分期及淋巴结转移有关。MUC4和ErbB2基因蛋白的高表达与NSCLC的发生有密切关系,二者在NSCLC发生发展中的作用有相关性。

Objective To investigate the expression,clinical significance and their association of mucin 4（MUC4） and tyrosine kinase receptor（ErbB2） in non-small cell lung cancer（NSCLC）.Methods The expressions of MUC4 and ErbB2 were detected by immunohistochemistry of SP in 70 cases of NSCLC（NSCLC group）and 30 cases of normal lung tissues near the tumor（control group）.Results The positive rates of MUC4（22.9%） and ErbB2（47.1%） expression were higher in NSCLC than those in normal lung tissues near the tumor significantly（P〈0.05）.The positive rates of MUC4 and ErbB2 expression increased with the decrease of pathological differentiation in NSCLC（P〈0.05）;The positive rates of MUC4 expression in clinical stage of Ⅲ-Ⅳ were significantly higher than those in stage of Ⅰ-Ⅱ（P〈0.05）,meanwhile the positive rates of ErbB2 expression in clinical stage of Ⅲ-Ⅳ were higher than those in stage of Ⅰ-Ⅱ.The positive rates of MUC4 and ErbB2 expression in NSCLC with lymph node metastasis were significantly higher than those without lymph node metastasis（P〈0.05）.Conclusion The over-expression of MUC4 was significantly related to pathology stage,clinical stage and lymph nodal metastasis in patients with NSCLC.MUC4 and ErbB2 might be specific tumor markers of NSCLC,and had correlation in the occurrence and development of NSCLC.