揭阳市RhD(-)稀有血型库的建立及临床意义

Establishment of RhD(-)blood type information system in Jieyang City and its clinical significance

目的:建立揭阳市无偿献血者RhD(-)稀有血型检索库,并探讨其临床意义。方法:采用微板法对揭阳市17542名无偿献血者RhD血型抗原进行初筛检测,对血型初筛RhD阴性标本再用3个不同批号的IgG抗-D抗体试剂进行间接抗人球蛋白(IAT)试验确认,初筛和确认试验均阴性者则为RhD阴性血型。同时检测RhD阴性者的ABO血型以及Rh血型的表现型。结果:所有献血者经筛选和确认共有66名RhD阴性血型,占0.38%,其中A型血最多,表现型以ccdee为主。将献血者的资料和检测的结果输入计算机,建立揭阳地区RhD(-)稀有血型检索库。结论:RhD(-)稀有血型检索库的建立对需要输血的RhD(-)患者有重大作用,能够保证同型输血,对防止Rh血型不合的输血引起的急性溶血反应及Rh血型不合的妊娠引起的新生儿溶血病具有重要的意义。

Objective：To establish RhD（-）blood type information system in Jieyang City,and analyze its clinical significance.Method：The RhD blood group antigens of 17542 volunteers of blood donors were detected by micro plate method.The negative results of RhD blood group antigen detected by indirect antiglobulin test（IAT）were confirmed using IgG anti-D antibodies reagents with three different lot numbers.The ABO group type and the phenotype of Rh were detected too.Result：There were 66 cases of RhD（-）blood group in all the donors,with percentage of 0.38%.Then the information of the RhD（-）donors were input into computer to establish the information system.Conclusion：The RhD（-）blood group information system would be very important to the patients who are the same group type and need blood transfusion.It could play an important role in preventing the acute hemolytic transfusion reactions and the hemolytic disease of newborns because of Rh blood group incompatibility.