兔骨髓基质细胞与聚DL-乳酸／羟基磷灰石复合异位成骨的实验研究

Osteogenic Potential of the.Composite of Bone Marrow Stromal Cells and PDLLA/HA in Vivo

目的 探讨骨髓基质细胞（BMSCs）与聚DL-乳酸/羟基磷灰石（PDLLA/HA）复合后异位成骨的生物学机制,了解其用于骨组织工程支架材料的可行性.方法 将BMSCs与PDLLA/HA复合物植入动物皮下后进行大体观察、组织学染色,观察其成骨过程.结果 一定浓度的BMSCs与PDLLA/HA复合植入动物皮下可产生软骨和骨组织,而单纯材料组未见软骨或骨形成.结论 PDLLA/HA复合BMSCs体内植入后能形成软骨或骨,并随植入时间的延长,软骨或新骨形成增多,PDLLA/ HA可用于骨组织工程的支架材料.

Objective To study the osteogenic mechanism of the composite of bone marrow stromal cells（BMSCs） and PDLLA/HA in Vivo and explore their possibility applied as the scaffold material of bone tissue engineering. Methods The composite of BMSCs and PDLLA/HA was implanted in subcutaneous sites in rabbits. All implants were examined by gross observation and underwent histological examination.Results Cartilage and bone are observed in the composites of the BMSCs and PDLLA/HA material in rabbit models.There was not any cartilage and bone formed at control group. Conclusions Cartilage and bone formed after PDLLA/HA composited with BMSCs implanted,cartilage and new bone formed much more followed with time.PDLLA/HA could be used as a scalfold of bone tissue engineering.