摘要
目的:建立柱前衍生化HPLC法测定左卡尼汀中光学异构体的含量。方法:左卡尼汀样品通过与衍生化试剂发生衍生化反应,用C18柱,以三乙胺缓冲溶液和四氢呋喃作为流动相,进行梯度洗脱,将左卡尼汀与其光学异构体分离;采用荧光检测器,激发波长234 nm,发射波长360 nm。结果:右卡尼汀在0.3～1.6μg.mL-1范围内线性关系良好,线性方程为Y=0.0173X+0.073 9r,=0.999 1,平均回收率为100.6%,RSD为1.97%(n=9)。结论:本法可用于检测左卡尼汀中光学异构体的含量,方法简便、准确、重现性好。
Objective： To develop a Pre-column derivatization HPLC method for the determination of D-carnitine in L-carnitine. Methods： Samples of L-carnitine were derivatized with （ ＋ ） MNPC and analyzed on Cls column by fluorescence detector （excitation wavelength 234 nm, emission wavelength 360 nm） using triethylamine buffer and tetrahydrofuran as the mobile phase with gradient elution. Results： The linear range of D-carnitine is 0. 3 - 1.6μg·mL-1 （ Y= 0. 017 3X ＋0. 073 9, r =0. 999 1 ）. The average recovery was 100. 6% with RSD 1.97% （n = 9）. Conclusion： The method is appropriate for the determination of D-earnitine in L-earnitine. The method is simple, accurate and reproducible.
出处
《中国药品标准》
CAS
2011年第5期381-384,共4页
Drug Standards of China
基金
国家重大科技专项(2009zx093132
008zx09401-004)
江苏省企业院士工作站资助项目(BM2009566)
