黄芪丹参药对及其有效组分对UUO大鼠肾组织JAK/STAT信号通路的影响

Effect of Astragalus and Salvia′s Effective Components and Their Compatibility on JAK/STAT Pathway

目的:观察黄芪丹参药对及其有效组分对单侧输尿管梗阻(UUO)大鼠肾组织JAK/STAT通路的影响。方法:66只SD大鼠随机分为7组,即正常对照组、模型组、黄芪丹参药对颗粒剂配伍组、黄芪丹参药对组分配伍组、丹参总酚酸组、黄芪总皂苷组、福辛普利组。各组给予相应药物治疗,观察黄芪丹参药对及其有效组分对单侧输尿管梗阻(UUO)大鼠尿β2微球蛋白、肾组织病理变化以及肾组织JAK/STAT通路的影响。结果:各治疗组肾脏组织病理变化均有不同程度的改善;黄芪丹参药对组分配伍能降低单侧输尿管梗阻(UUO)大鼠尿β2-MG(P<0.05),与福辛普利组相当,其余各治疗组有下降趋势,但与模型组比较,差异无显著性;黄芪丹参药对及其有效组分可降低模型大鼠肾组织JAK、STAT1、STAT3蛋白的表达,以组分配伍及颗粒配伍作用显著,与福辛普利相当,黄芪总皂苷作用不显著,其余各治疗组均有不同程度的降低作用。结论:黄芪丹参药对及其组分能保护单侧输尿管梗阻的肾小管功能,一定程度改善肾纤维病理,其机制可能与其干预UUO大鼠肾组织JAK/STAT信号通路有关。

Objective：To study the effect of Astragalus and Salvia′s effective components and their compatibility on JAK/STAT pathway of rats′ renal fibrosis.Methods：66 SD rats were randomly divided into 7 groups：normal group,model group,fosinopril group,salvianolic acids group,astragalus saponins group,granules compatibility of Astragalus and Salvia group,components combination of Astragalus and Salvia group.The variation of β_2-microglobulin（β_2-MG）,the changes of renal pathology and JAK/STAT pathway were observed.Results：The changes in renal pathology of treatment groups had different degrees of improvement;Astragalus and Salvia could reduce the urinary β_2-MG of unilateral ureteral obstruction（UUO） rat（P0.05）,which was equal with fosinopril group.The rest of the treatment groups decreased especially fosinopril group while the difference was not significant when compared with the model group.Astragalus and its effective components could reduce the expression of renal tissue JAK,STAT1,STAT3 protein,among which fosinopril group and granules compatibility of Astragalus decreased significantly.Astragalus saponins group was not obvious,and the rest of the treatment group had significantly minor effect.Conclusion：Astragalus and Salvia′s effective components and their compatibility may protect renal tubular function in unilateral ureteral obstruction,which may interfere with UUO rat kidney with JAK/STAT signaling pathway.