摘要
目的:优选淫羊藿多糖脂质体(EPSL)制备的最佳条件。方法:采用薄膜分散超声法制备EPSL,以包封率和载药量为指标,以脂药比、膜材比、超声时间和载药温度为因素水平,用L9(34)正交试验对制备条件进行优选,并用鱼精蛋白法测定EPSL的包封率。结果:制备EPSL的最佳条件为:脂药比30∶1,膜材比4∶1,超声时间10 min,载药温度40℃。结论:采用最佳条件制备的EPSL,包封率和载药量较高。
Objective: To optimize the preparation conditions of epimedium polysaccharide liposome(EPSL).Methods: EPSL was prepared by membrane distribution-supersound method.In preparation of EPSL test,an orthogonal L_9(34) test was used to optimize the preparation conditions,with four factors of ratio of drug to lipid,ratio of lecithin to cholesterol,ultrasonic time and water bath temperature and two indexes of encapsulation efficiency and drug-loading rate.The encapsulation efficiency of EPSL was determined by protamine method.Results: Optimal preparation condition of EPSL was that ratio of drug to lipid,ratio of lecithin to cholesterol,ultrasonic time and water bath temperature were 1∶30,4∶1,10 min and 40℃ respectively.Conclusion: EPSL prepared under Optimal conditions has the property of high encapsulation efficiency and drug-loading rate.
出处
《中药材》
CAS
CSCD
北大核心
2011年第9期1429-1433,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(30901085)
教育部科学技术重点项目(108153)
关键词
淫羊藿多糖脂质体
制备
正交试验
鱼精蛋白法
Epimedium polysaccharide liposome
Preparation
Orthogonal test
Protamine method