软肝化坚颗粒对肝纤维化模型C57小鼠肝脏PDGF-B表达的影响

Effect of Ruangan Huajian granule on hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis

目的观察软肝化坚颗粒对肝纤维化模型C57小鼠肝组织血小板衍生生长因子-B(PDGF-B)表达的影响。方法 50只C57小鼠被随机分为5组,每组10只。采用四氯化碳腹腔注射制造小鼠肝纤维化模型,通过免疫组织化学染色方法检测各实验组肝组织中PDGF-B的表达,对不同剂量软肝化坚颗粒进行实验对比,同时进行肝组织炎症评分(G)和纤维化评分(S)。结果各实验组肝组织PDGF-B表达、炎症和纤维化评分与正常对照组比较,差异均具有统计学意义(P<0.05);模型对照组肝组织PDGF-B表达、炎症和纤维化评分明显高于软肝化坚颗粒低剂量组、高剂量组和秋水仙碱组,差异均具有统计学意义(P<0.05);软肝化坚颗粒低剂量组、高剂量组和秋水仙碱组肝组织PDGF-B表达、炎症和纤维化评分差异无统计学意义(P>0.05)。结论软肝化坚颗粒可能通过降低C57小鼠肝组织PDGF-B的表达,进而减轻肝纤维化程度和肝组织损伤。

Objective To observe the effect of Ruangan Huajian granule on hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis.Methods Fifty C57 mice were randomly divided into five groups（10 mice in each group）,i.e.normal control group,control model group,low-dose Ruangan Huajian granule model group,high-dose Ruangan Huajian granule model group and colchicine model group.C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4.The expression of PDGF-B in liver tissue of mice was observed by immune histochemical technique.Compare the expression of PDGF-B in different Ruangan Huajian granule dosage group with other experimental groups.The scores of inflammation grade（G） and fibrosis stage（S） in liver tissue were also analyzed.Results The expression of PDGF-B and the scores of G and S in model groups were significantly higher than those in normal control group（P0.05）,and that of model control group were significantly higher than Ruangan Huajian granule low-dose model group,Ruangan Huajian granule high-dose model group and colchicine model group（P0.05）.There was no significant difference among Ruangan Huajian granule low-dose model group,Ruangan Huajian granule high-dose model group and colchicine model group（P0.05）.Conclusion Ruangan Huajian granule may alleviate the degree of liver inflammation and fibrosis through reducing hepatic expression of PDGF-B in C57 mice with experimental hepatic fibrosis.