新西兰大白兔骨膜下组织工程骨异位成骨的实验研究

Experimental study of ectopic bone formation of engineered bone constructs under the periosteum of New-Zealand rabbits

目的:利用新西兰大白兔骨髓基质干细胞构建组织工程骨,研究其在股骨骨膜下异位成骨的可行性。方法:选取3月龄的清洁级雌性新西兰大白兔,体重3kg,取其骨髓基质干细胞诱导为成骨干细胞,扩增后接种到β-磷酸三钙生物陶瓷颗粒中,构建的组织工程骨种植到股骨骨膜下,3个月后实验动物血管内灌注凝胶墨汁,通过光学显微镜直接检测组织工程骨的血供和成骨结果。结果:16个标本中的12个植入的组织工程骨颗粒均良好固定在骨膜下并被骨膜包围,组织工程骨中有大量血管和新生骨形成,骨组织结构相对紊乱,不同于正常骨组织结构排列规则,血管分布均匀。4例取材时发现植入物游离于骨膜外,大部分材料被吸收,残留植入物体积明显小于骨膜下成骨,未见明显的骨组织形成,血供情况欠佳。股骨骨膜下组织工程骨颗粒80%贴附牢固,成骨良好,新骨内有大量血管长入。结论:组织工程骨可以在骨膜下获得良好的异位成骨。

Objective：To study the feasibility of ectopic bone formation for engineered bone constructs with bone marrow stromal cells（BMSCs）.Methods：BMSCs were obtained from 3-month female New-Zealand rabbits with weight of 3 kg,induced to osteogenitor cell,were expanded by culture and then seeded into the porous β-tricalcium phosphate（β-TCP） particles.Engineered bone constructs were implanted under the periostem of rabbit femur.Samples were retrieved and examed after 3 months.Blood vessels and osteoblast were examined through optical microscope.Results：Twelve implanted engineered bone particles of 16 samples were fixed well under periosteum and rounded by periosteum.There were a lot of vessels and new bone in engineered bone.The structure of bone was disorder;the vessels arranged equally.Four cases found implanted bone freed outside of periosteum,lots of implanted material were absorbed,the volume of residual was less than osteogenesis,and lack of blood vessel.80% engineered bone constructs attached to the femur under the periostem very well,osteogenesis was fine and vessels were growed into new bone.Conclusion：Engineered bone can obtained good ectopic bone under the periostem.