干细胞标志物Nanog、Oct-4、SOX-2表达与结肠癌术后复发转移关系

Correlation of expression of Nanog, Oct-4 and SOX-2 with tumor metastasis in colonic cancer

目的:探讨结肠癌患者肿瘤组织中Nanog、Oct-4、SOX-2表达情况及其对远处转移的预测作用.方法:采用免疫组织化学方法检测经手术切除的80例结肠癌术后标本中Nanog、Oct-4、SOX-2表达情况,并且对三种指标的表达同肿瘤分化程度、分期和术后复发转移关系进行分析.结果:80例患者有35例出现复发转移,SOX-2、Oct-4、Nanog在转移组患者中表达率分别为48.57%(17/35)、51.43%(18/35)、60%(21/35),在非转移组中表达率分别为17.78%(8/45)、13.33%(6/45)、26.67%(12/45)差异有统计学意义.原发灶分化程度仅同Nanog表达差异有统计学意义(P=0.001).但是三指标表达同T分期和N分期没有明显联系.三者均为表达阳性其转移率明显高于均为阴性转移率,生存分析显示不同表达状态其远处转移出现时间差异有统计学意义(P=0.0001).结论:组织中Nanog、Oct-4、SOX-2表达同结肠癌术后复发转移发生相关,联合检测有助于评估肿瘤转移情况.

AIM： To investigate the expression of Nanog, Oct4 and SOX-2 in colonic carcinoma and to analyze their relationship with tumor metastasis.METHODS： Immunohistochemistry was used to evaluate the expression of Nanog, Oct-4 and SOX-2 in 80 colonic cancer surgical specimens. The correlation of Nanog, Oct-4 and SOX-2 expression with tumor histological differentiation,T stage, N stage and metastasis was analyzed.RESULTS： Thirty-five patients had metastasis. The positive rates of SOX-2, Oct-4 and Nanog expression were significantly higher in the me- tastasis group than in the non-metastasis group （48.57% vs 17.78%, 51.43% vs 13.33%, 60% vs 26.67%, all P 〈 0.05）. Nanog expression was significantly correlated with differentiation （P = 0.001）, but expression of Nanog, Oct-4 and SOX-2 was not associated with T stage or N stage. Metastatic rate was higher in patients with positive expression of the three markers than in those with negative expression. Survival analysis showed that the time to develop metastasis was significant different among patients with differential expression of the three markers （P = 0.0001）.CONCLUSION： The expression of Nanog, Oct-4 and SOX-2 in tumor tissue can predict the post-operative metastasis of colonic cancer. Nanog, Oct-4 and SOX-2 are promising predictors for metastasis of colonic cancer.