胃良恶性病变组织中TAG72和GRP94表达及其临床病理意义

Expression of tumor-associated glycoprotein 72(TAG72) and glucose-regulated protein 94(GRP94) and their clinicopathological significances in benign and malignant lesions of stomach

目的研究胃良恶性病变组织中肿瘤相关糖蛋白72(TAG72)和葡萄糖调节蛋白94(GRP94)表达水平及其临床病理意义。方法将49例胃癌,20例癌旁组织,36例淋巴结转移灶及80例不同类型胃良性病变患者手术切除标本或胃镜活检标本行常规石蜡切片,采用免疫组化法检测TAG72和GRP94的表达。结果胃癌组织TAG72和GRP94表达阳性率高于癌旁组织及各类型胃良性病变(P<0.05或P<0.01);TAG72和/或GRP94阳性表达的癌旁组织及胃良性病变黏膜上皮均呈轻至重度不典型增生;胃癌原发灶与相应淋巴结转移灶之间TAG72和GRP94表达阳性率均无统计学差异(均P>0.05);浸润深度T1+T2者、无区域淋巴结转移及无远处器官转移者TAG72和GRP94表达阳性率均明显低于浸润深度T3+T4者、区域淋巴结转移及远处器官转移者(P<0.05或P<0.01);组织学分级II级及N1站淋巴结转移者TAG72和GRP94表达阳性率低于组织学III+IV级及N2+N3站淋巴结转移者,但差异均无统计学意义(均P>0.05)。结论 TAG72和GRP94表达水平对胃癌发生、进展和临床生物学行为方面可能有促进作用,TAG72和/或GRP94阳性表达的胃癌预后不良。

Objective To study the expression of tumor-associated glycoprotein 72（TAG72） and glucose-regulated protein 94（GRP94） and their clinicopathological significances in benign and malignant lesions of the stomach. Methods The conventional paraffin-embedded sections were prepared from the surgical resected or gastroscopic biopsy specimens that included 49 cases of gastric cancer tissues,20 cases of peritumoral tissues,36 cases of metastastic foci in lymph nodes and 80 cases of benign lesions of different types.Immunohistochemical staining was performed to determine the expression level of TAG72 and GRP94. Results The positive rates of TAG72 and GRP94 expression in gastric cancer were significantly higher than those in peritumoral tissues and different types of benign lesions（P0.05 or P0.01）.The peritumoral tissues and benign lesions with positive expression of TAG72 and/or GRP94 all showed mild to severe degrees atypical hyperplasia of mucosal epithelia.The positive rates of TAG72 and GRP94 expression showed no significant differences between the primary foci and the corresponding metastatic foci in lymph nodes（both P0.05）.The positive rates of TAG72 and GRP94 expression in the cases of tumor invasion depth T1＋T2 and no metastasis to regional lymph nodes or distant organs were significantly lower than those of invasion depth T3＋T4,metastasis of regional lymph nodes or distant organs（P0.05 or P0.01）.The positive rates of TAG72 and GRP94 expression in the cases of histological grade II and N1 lymph nodes involvement were lower than those of histological grade III＋IV and N2＋N3 lymph nodes involvement,but both had no significant difference（both P0.05）. Conclusions The expression level of TAG72 and/or GRP94 may play a promoting role in the occurrence,development and biological behaviors of gastric cancer,and the positive expression of TAG72 and/or GRP94 in gastric cancer patients predicts a poor prognosis.