胃癌腹膜转移相关基因的检测

Detection of genes related to peritoneal metastasis from gastric cancer

目的筛选与胃癌腹膜转移相关的基因,为研究胃癌腹膜转移的机制提供参考。方法建立裸鼠人胃癌细胞株SGC-7901皮下移植瘤模型,鼠间传3代后移植小瘤块到裸鼠胃壁,收集原发灶和转移灶标本。采用基因芯片技术筛选胃癌原发灶和转移灶间差异表达的基因,并用RT-PCR法检验基因芯片的结果。结果共建立胃癌原位移植裸鼠15只,胃壁成瘤率为100%(15/15),腹膜转移发生率为40.0%(6/15)。芯片杂交分析筛选出192个上调基因和139个下调基因;RT-PCR验证高表达的再生基因IV(Reg IV),结果与基因芯片检测情况相符。结论胃癌原发灶与腹膜转移灶之间存在差异表达的基因,其中部分基因可能在胃癌的腹膜转移中起了促进作用。

Objective To screen out the genes related to peritoneal metastasis from gastric cancer,so as to provide clues to the underlying mechanisms of peritoneal metastasis of gastric cancer. Methods The models of subcutaneously transplanted human gastric cancer in nude mice were established using SGC-7901 cell line.After three subcutaneous passages of the original xenograft through mice,the small dissected pieces of solid tumor were introduced into the stomach wall of nude mice.Tumor tissues from the original transplant sites and metastases were collected.Gene chip technology was used to determine the differentially expressed genes between the original tumor and metastasis,and then RT-PCR was performed to validate the result of the gene chip analysis. Results Fifteen orthotopic transplant nude mice with gastric cancer were established,of which,the tumor formation rate in stomach wall was 100%（15/15） and the incidence of peritoneal metastases was 40%（6/15）.There were 192 up-regulated genes and 139 down-regulated genes as shown by gene chip hybridization analysis.The highly expressed regenerating gene IV（Reg IV） was tested by RT-PCR and the result was consistent with that of the gene chip analysis. Conclusions There are differentially expressed genes between the primary tumor and metastases of gastric cancer,some of which may possibly play an important role in peritoneal metastasis of gastric cancer.