阿托品滴眼液制作兔干眼模型的研究

Establishment of a rabbit dry eye model by using sulfate atropine eye drops

目的探讨阿托品滴眼液制作兔干眼模型优缺点。方法健康新西兰大白兔12只,随机分为两组:正常组、阿托品组,其中正常组2只兔(4只眼)不接受任何处理,正常饲养14 d和30 d后分别处死1只取泪腺、哈氏腺及角膜行光镜检查,阿托品组10只兔(20只眼)双眼滴用1%硫酸阿托品滴眼液。对阿托品组分别于造模前及造模后第1、3、5、7、11、14天,检查角膜荧光素染色、SchirmerⅠ试验。阿托品造模组点药后14 d后处死取泪腺、哈氏腺行光镜检查,取泪腺及角膜行光镜检查。结果阿托品组用药后11 d内角膜荧光素染色评分增加(P<0.05),但用药14 d后角膜荧光素染色与用药前相比,其差异无显著性意义(P>0.05);用药后第3～11天,基础SchirmerⅠ试验值较用药前显著性减少(P<0.05);用药后14 d检查泪腺、哈氏腺的形态与正常组比较无明显差别。结论阿托品滴眼液点眼制作的干眼症模型经济快速,但不能持久。

Objective To investigate the characteristics of rabbit dry eye model by using sulfate atropine eye drops.Methods 12 healthy New Zealand rabbits were randomly divided into two groups： 2 rabbits（4 eyes） in normal group with no treatment,and the atropine group.At the 14th day and 30th day,one rabbit in the control group was sacrificed.The lacrimal gland,and Harderian gland was collected,and corneal light microscopic examination was performed.10 rabbits（20 eyes） in the atropine group were treated with eye drops of 1% sulfate atropine.Corneal fluorescein staining and Schirmer Ⅰ test was performed in the atropine group before modeling and at 1,3,5,7,11,14,days after modeling.At the 14th day,rabbits in the atrophine group were sacrificed and t the lacrimal gland,Harderian gland were collected.Results Atropine group： corneal fluorescein staining score increased at the 11th day after treatment（P〈0.05）,it was not significantly different at the 14th day after treatment（P〈0.05）;at the 3rd day after treatment,Schirmer Ⅰ test values significantly increased（P〈0.05）;at the 14th day after treatment,microscopic examination of lacrimal gland,Harderian gland is not significantly different than the normal group.Conclusion Rabbit dry eye model by using sulfate atropine eye drops is rapid and economy,but can not be sustained.