鼠巨细胞病毒感染小鼠骨髓单个核细胞亚群的研究

Infection of the Mononuclear Cell Subpopulations in Murine Bone Marrow with Murine Cytomegalovirus

本研究旨在探索鼠巨细胞病毒(MCMV)在小鼠骨髓单个核亚群细胞中的感染特性。采用免疫磁珠分选法(MACS)分选小鼠骨髓单个核亚群细胞,包括lin+细胞、lin-细胞、lin-CD117+和lin-CD117-细胞;用MCMV攻击分选的各组分细胞并以细胞因子和诱导剂诱导细胞分化,最后检测病毒核酸及相关蛋白。结果表明:在MCMV感染的lin+细胞中可检测到病毒DNA和立即早期基因的转录产物及其蛋白,而在感染的lin-细胞中则未检测到任何病毒产物。通过添加细胞因子GM-CSF、rhEPO或佛波酯,则可以在感染的lin-细胞中检测到MCMV的立即早期和早期基因转录产物以及蛋白表达。在lin-CD117+和lin-CD117-细胞中也未检测到病毒基因转录产物,但MCMV感染可抑制lin-CD117+造血干/祖细胞集落的形成以及下调其表面标志CD117抗原的表达。结论:MCMV可在小鼠骨髓单个核亚群细胞中潜伏感染;小鼠骨髓中具有原始干/祖细胞性质的细胞群对MCMV不易感,但MCMV感染可对这些细胞的功能产生抑制作用,可使细胞表面标志表达下调。

This study was aimed to explore the infection characteristics of murine mononuclear cell subpopulations in bone marrow with murine cytomegalovirus（MCMV）.Subpopulations of mononuclear cells,including lin＋、lin-、lin-CD117＋ and lin-CD117-cells,were infected with MCMV after being separated by MACS,and induced to differentiation by adding cytokines or inducer,then nucleic acid and proteins were detected.The results indicated that the MCMV DNA,IE transcrip and IE protein could be detected in the lin＋ cells infected with MCMV;no virus products were detected in infected lin-cells without adding any stimulating factors,while IE and E transcrips and proteins were detected after adding GM-CSF、rhEPO or phorbol ester in the lin-cells infected with MCMV.Furthermore,no IE or E gene transcrips were detected in the lin-CD117＋ and lin-CD117-cells,but the cell colony formation of lin-CD117＋ hematopoietic stem and progenitor cells was inhibited after MCMV infection and expression of CD117 antigen on cell surface of the lin-cells was downregulated.It is concluded that MCMV can latently infect subpopulations of mononuclear cells in the murine bone marrow.Cells which are of characteristics of primitive stem and progenitor cells are not susceptible to MCMV,but infection of these cells with MCMV can inhibit functions of cells and downregulate the expression of antigen on cells surface.