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不同淋巴道转移潜能小鼠肝癌细胞系中Toll样受体2基因的表达

TLR2 gene expression level in mouse hepatocellular carcinoma cell lines with differently lymphatic metastasis potentials
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摘要 目的探讨TLR-2基因表达水平与小鼠肝癌淋巴道转移中的关系。方法采用RQ-PCR方法检测TLR-2基因在肝癌细胞系H22、淋巴道低转移潜能的小鼠肝癌细胞系Hca-P和高转移潜能的小鼠肝癌细胞系Hca-F中的表达水平。结果 TLR-2基因在H22、Hca-P和HcaF细胞系基因表达分别为(1.08±0.25)×10-3、(2.14±0.42)×10-3、(4.31±0.62)×10-3,差异有统计学意义(P<0.01),其随转移潜能的增高,表达水平增加。结论 TLR-2基因表达水平增高提示其在肝癌的淋巴道侵袭转移中起作用,有可能成为预测肝癌预后新的靶点。 Purpose To study Toll-like receptor 2（TLR-2） gene expression level in mouse hepatocellular carcinoma cell lines with differently lymphatic metastasis potentials and to discuss there roles in lymphatic metastasis.Methods Using real-time quantitative PCR,TLR-2 gene expression level was detected in mouse hepatocellular carcinoma cell lines,which were H22,Hca-P with low lymphatic metastasis potential and Hca-F with high lymphatic metastasis potential.Results TLR-2 gene mRNA expression in H22,Hca-P and Hca-F cell lines were（1.08±0.25）×10-3,（2.14±0.42）×10-3,（4.31±0.62）×10-3（P0.01）,respectively.TLR-2 gene mRNA expression levels was increased with metastasis potential increasing.Conclusion TLR-2 genes may play an important role in lymphatic metastasis of hepatocellular carcinoma.It could be a potential marker to predict metastasis and a target to anti-metastasis intervention.
作者 冀学宁 王若雨 李梅 范学军 葛日光 吕申
机构地区 大连大学附属中山医院肿瘤科 大连医科大学附属二院实验中心
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2011年第10期1087-1089,1094,共4页 Chinese Journal of Clinical and Experimental Pathology
基金 大连市卫生科技基金(2006-73)
关键词 肝肿瘤 肝细胞癌 TLR-2基因 转移 liver neoplasms hepatocellular carcinoma TLR-2 genes metastasis
分类号 R73-37 [医药卫生—肿瘤] R735.7 [医药卫生—肿瘤]
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参考文献11

  • 1Tabeta K,Georgel P,Janssen E,et al.TLR9 and TLR3 as essential components of innate immune defense against mouse cytomegalovirus[J].Proc Natl Acad Sci USA,2004,101(10):3516-21.
  • 2Huang B,Zhao J,Li H X,et al.Toll-like receptors on tumor cells facilitate evasion of immune surveillance[J].Cancer Res,2005,65(12):5009-14.
  • 3Kelly M G,Alvero A B,Chen R,et al.TLR-4 signaling promotes tumor growth and paclitaxel chemoresistance in ovarian cancer[J].Cancer Res,2006,66 (7):3559-68.
  • 4Merrel M A,Ilvesaro J M,Lehtonen N,et al.Toll-like receptor 9 agonists promote cellular invasion by increasing matrix metalloproteinase activity[J].Mol Cancer Res,2006,4(7):437-47.
  • 5Szczepanski M,Stelmachowska M,Stryczynski L,et al.Assessment of expression of toll-like receptors 2,3 and 4 in laryngeal carcinoma[J].Eur Arch Otorhinolaryngol,2007,264(5):525-30.
  • 6龚志勇,李响,毛普德,王继忠,李虹,杨宇如.膀胱癌细胞钟声蛋白样受体表达与卡介苗诱导[J].中国肿瘤,2004,13(1):45-48. 被引量：5
  • 7Huang B,Zhao J,Shen S,et al.Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signalling[J].Cancer Res,2007,67(9):4346-52.
  • 8Chang Y J,Wu M S,Lin J T,et al.Induction of cyclooxygenase-2 overexpression in human gastric epithelial cells by helicobacter pylori involves TLR2/TLR9 and c-Src-Dependent nuclear factor-κB activation[J].Mol Pharmaco,2004,l66(6):1465-77.
  • 9Yoshioka T,MorimotoY,Iwagaki H,et al.Bacterial lipopolysaccharide induces transforming growth factor beta and hepatocyte growth factor through toll-like receptor 2 in cultured human colon cancer cells[J].J Int Med Res,2001,29(5):409-20.
  • 10赵文忠,刘艳君,朱平,刘洁,韩强涛,富宁.抗小鼠TLR-2胞外段B细胞识别表位抗体抑制肉瘤生长的初步研究[J].第一军医大学学报,2004,24(8):873-876. 被引量：2

二级参考文献20

  • 1[1]Tsuji S, Matsumoto M, Takeuchi O, et al. Maturation of human dendritic cells by cell wall skeleton of Mycobacterium bovis bacillus Calmette-Guerin:Involvement of Tolllike receptors[J].Infect Immunol,2000,68:6883-6890.
  • 2[2]Schilling JD,Mulvey MA,Vincent CD,et al. Bacterial invasion augments epithelial cytokine responses to Escherichia coli through a lipopolysaccharide-dependent mechanism[J].J Immunol,2001,166:1148-1155.
  • 3[3]Means TK, Jones BW, Schromm AB, et al. Defferential effects of a toll-like receptor antagonist on mycobacterium tuberculosis-induced macrophage responses [J].Immunol,2001, 166:4074-4082.
  • 4[4]Hoffmann JA,Kafaros FC,Janeway CA,et al. Phylogenetic perspective in innate immunity[J].Science, 1999,284:1313-1318.
  • 5[5]Anderson KV.Toll signaling pauhway in the innate immune response[J].Curr Opin Immunol, 2000,12:13-19.
  • 6[6]Jones BW, Means TK, Heldwein KA, et al. Different Tolllike receptor agonists induce distinct macrophage responses[J].J Leukoc Biol, 2001,69(6):1036-1044.
  • 7[7]Brightbill HD, Libraty DH, Krutzik SR, et al. Host defense mechanisms triggered by bacterial lipoproteins through Toll-like receptors[J].Science, 1999,295:732-736.
  • 8[8]Backhed F, Soderhall M, Ekman P, et al. Induction of innate immune responses by Escherichia coli and purified lipopolysaccharide correlate with organ-and cell-specific expression of Toll-like receptors within the human urinary tract[J].Cell Microbiol,2001,3:153-158.
  • 9[9]Cario E, Brown D, McKee M, et al. Commensal- associated molecular patterns induce selective toll-like receptortrafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium [J].Am J Pathol,2002,160(1):165-173.
  • 10Modlin RL, Brightbill HD, Godowski PJ. The toll of innate immunity on microbial pathogens[J]. N Engl J Med, 1999, 340(23):1834-39.

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临床与实验病理学杂志
2011年 第10期

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