异基因外周血造血干细胞供者采集术的护理

Nursing of Allogeneic Donor Peripheral Blood Stem Cell Collection

目的探讨供者外周造血干细胞采集术的护理,提供足够细胞数量的外周造血干细胞数,确保受者外周造血干细胞移植的成功。方法分析2006～2010年供者资料,其外周血干细胞动员方案为粒系集落刺激因子或加用粒单核系集落刺激因皮下注射,1次/d,连续4 d或5 d,白细胞水平升至30×109/L,应用COBE Spectra全血细胞单采机采集和分离供者的外周血干细胞,输给白血病受者,整过程实行全方位的护理。结果 107例供者共采集了128次外周造血干细胞,在总循环9000～12 000 ml情况下,采集的外周血干细胞中的WBC 80%以上,CD34浓度是采集前CD34浓度的5倍以上,安全输给107例受者,移植成功率99%。结论提供给供者从入院至采集结束的心理、饮食、基础护理及专业等全方位护理是采集成功的前提,是外周造血干细胞的移植成功的关键。

Objective To probe nursing of donor peripheral blood stem cell collection,to provide sufficient peripheral blood stem cells,and to ensure successful transplantation of peripheral blood stem cell of providers.Methods Data from recipients during 2006-2010 were analyzed.The peripheral blood stem cell mobilization regimen for recombinant human granulocyte colony stimulating factor（rhG-CSF） 300 μg,sc,1 day,4 d,or continuously 5 d,as a result,white blood cells rose to 30×109/L.Then by COBE Spectra apheresis unit of whole blood cell the patient＇s peripheral blood stem cells were collected and separated,and then input into the providers with leukemia.Throughout the entire process,a full range of care was conducted.Results 128 peripheral blood stem cells were collected from 107 donors.In the total cycle 9000-12 000 ml cases,80% or more WBC of peripheral blood stem cells were collected,and CD34 concentration more than 5 times than before collection.The cells were in security input into 107 recipients,and transplantation success rate was 99%.Conclusion Psychological,diet,basic care and a full range of professional care were provided for providers from the beginning to the end of the collection was a prerequisite for success,and was the key to success in transplantation of a peripheral blood stem cell.