雌激素和IFN-γ对去卵巢大鼠下丘脑中Bcl-2和Bax表达的影响

Effect of estrogen and IFN-γ on the expression of Bcl-2 and Bax in hypothalamus of ovariectomed rats

【目的】研究雌激素与γ-干扰素(IFN-γ)对下丘脑的协同保护作用。【方法】建立去卵巢(OVX)模型大鼠,然后将其分为摘除卵巢(OVX)组、假手术(SHAM)组、OVX+17-β雌二醇(E2)组、OVX+E2+50IU IFN-γ组、OVX+E2+200IU IFN-γ组和OVX+E2+800IU IFN-γ组共6组,分别在处理后的0,2,4,6,8周,用免疫组织化学SP法对各组大鼠下丘脑中凋亡相关蛋白Bcl-2和Bax的表达进行检测。【结果】OVX组大鼠下丘脑中Bcl-2的相对表达量随时程逐渐减少,4周后最低,6周后又逐渐上升,随后维持在一定的水平,Bax表达量的变化趋势则与之相反;OVX组Bcl-2和Bax的表达量与SHAM组差异极显著(P<0.01)。OVX+E2组大鼠下丘脑中Bcl-2的相对表达量增加,Bax的相对表达量降低,均与OVX组差异显著(P<0.05)。OVX+E2+200IU IFN-γ组Bcl-2的相对表达量极显著高于OVX组,显著高于OVX+E2组,Bax的总体变化趋势与OVX+E2组一致;OVX+E2+50IU IFN-γ组和OVX+E2+800IU IFN-γ组Bcl-2和Bax的相对表达量与OVX+E2组差异不显著(P>0.05)。【结论】雌激素的缺乏增强了下丘脑神经元对凋亡的敏感性,而外源生理剂量E2与适当剂量的IFN-γ,在一定时期内对这种凋亡有重要的保护作用。

【Objective】 The research was to study the synergy effect of estrogen and interferon-γ（INF-γ） on protecting the hypothalamus nucleus.【Method】 The immunohistochemical SP method was used to study the expressions of Bcl-2 and Bax in hypothalamus of the ovariectomized（OVX） group,the SHAM group,the OVX＋17-β estrogen（E2） group,OVX＋E2＋50 IU IFN-γ group,OVX＋E2＋200 IU IFN-γ group and OVX＋E2＋800 IU IFN-γ group.【Result】 The main results were as follows：After ovariectomization,relative expressions of Bcl-2 positive productions（μ2） tapered with the course and reached the minimum after 4 weeks concurrently.The μ2 of Bax increased and reached the maximum after 4 weeks,which had significant difference with the SHAM group（P0.01）.OVX＋E2 group,the μ2 of Bcl-2 increased and had remarkable difference with OVX group（P0.01）,the μ2 of Bax decreased and had remarkable difference with OVX group（P0.01）;The μ2 of Bcl-2 of OVX＋E2＋200 IU IFN-γ group had different changes with OVX group（P0.01）,OVX＋E2 group（P0.05）,and SHAM group（P0.05）.The total tendency of the μ2 of Bax had very significant difference with OVX group（P0.01） in the nucleus which the μ2 of Bax decreased.【Conclusion】 The sensibility of the neurons in hypothalamus to apoptosis was enhanced by estrogen deficiency,and physiological dose E2 combined with proper-dose IFN-γ had important protecting effect on the apoptosis within a certain period.