柴胡疏肝散对抑郁模型大鼠海马CA3区神经元的影响

Effect of Chaihu Shugan Powder on Apoptosis in Hippocampal CA3 Area of Depressive Rats

目的观察柴胡疏肝散对抑郁模型大鼠海马CA3区神经元凋亡的影响。方法 60只4～5月龄的SD雄性大鼠随机分为正常组、模型组、西药组和中药组,共4组,每组15只。采用孤养和慢性轻度不可预见性应激刺激制备抑郁大鼠模型。利用敞箱实验、糖水偏爱度和体重检测等指标评定大鼠行为学改变,并用电镜观察大鼠脑海马CA3区超微结构变化,流式细胞术检测CA3区的细胞凋亡数目。结果造模21 d后,与正常组相比,模型组水平运动得分、垂直运动得分、体重和糖水偏爱度明显降低(P<0.01或P<0.001);与模型组比较,西药组和中药组水平运动得分、垂直运动得分、糖水偏爱度与体重均明显升高(P<0.05或P<0.001);流式细胞检测结果显示,与正常组相比,模型组海马CA3区凋亡细胞平均数明显增加(P<0.05);与模型组相比,西药组、中药组海马CA3区凋亡细胞平均数明显减少(P<0.05)。同时,电镜观察结果也显示,海马凋亡细胞主要集中在模型组、西药组和中药组。结论柴胡疏肝散的抗抑郁作用可能与拮抗海马CA3区神经元凋亡有关。

Objective To investigate the effect of Chaihu Shugan Powder on apoptosis in hippocampal CA3 area of depressive rats.Methods 60 male Sprague-Dawley rats with 4 ～ 5 months were randomly divided into four groups and 15 rats in every group： normal group,model control group,western medicine treatment control group and Chinese medicine group.After twenty-one days,behavior changes in rats were detected through open-field test,sugar consumption test and body weight;ultrastructural changes and apoptosis in hippocampal CA3 area of brain were observed respectively with electron microscope and flow cytometry.Results On the 21st day of model replication,compared with normal control group,the scores of horizontal activity and vertical activity,weight and sugar preference degrees in model group continued to decrease significantly（P0.01 or P 0.001）;compared with model group,western medicine group and Chinese medicine group got significantly higher scores of horizontal activity and vertical activity,weight and sugar preference degrees（P0.05 or P0.001）.Flow cytometry test results showed that model group had a marked increase in average number of apoptotic cells in hippocampal CA3 area compared with normal control group（P0.05）;Compared with model group,the average number of apoptotic cells in hippocampal CA3 area of western medicine group and that of Chinese medicine group decreased significantly（P0.05）.At the same time,electron microscope results also showed that apoptotic cells in the hippocampus mainly concentrated in the model group,western medicine group and chinese medicine.Conclusion Chaihu Shugan Powder can improve the depressive symptoms of experimental animals cell apoptosis in hippocampus of the brain tissue.