宾川獐牙菜化学成分研究

Study on Chemical Constituents of Swertia binchuanensis

目的研究宾川獐牙菜(Swertia binchuanensis)的化学成分,寻找生物活性物质。方法分别用石油醚,醋酸乙酯,氯仿和正丁醇依次萃取宾川獐牙菜乙醇提取物,再经MCI、硅胶和D101等柱层析进行分离纯化。根据化合物的理化性质和UV、MS、1H-NMR、13C-NMR等波谱手段鉴定其结构。结果从宾川獐牙菜的脂溶性部分分离得到4个化合物,分别鉴定为1-羟基-3,5-二甲氧基酮(1-hydroxy-3,5-dimethoxyxanthone,Ⅰ),1,5,8-三羟基-3-甲氧基酮(bel-lidifodin,Ⅱ),齐墩果酸(oleanolic acid,Ⅲ),獐牙菜苦苷(swertamarin,Ⅳ)。结论 4个化合物皆为首次从宾川獐牙菜中分离得到。

Objective To study the chemical constituents of Swertia binchuanensis for finding active compounds.Methods The ethanol extracts of S.binchuanensis was extracted with petroleum ether,ethyl acetate,chloroform and butanol,respectively.The constituents were isolated and purified by coloumn chromatograph with MCI,silica gel and D101.Their structures were identified on the basis of spectral analysis such as UV,MS,1H-NMR and 13C-NMR.Results Four compounds were isolated and identified as 1-hydroxy-3,5-dimethoxyxanthone（I）,bellidifodin（II）,oleanolic acid（III） and swertamarin（IV）.Conclusion All compounds were isolated from Swertia binchuanensis for the first time.