光枝勾儿茶高效液相色谱指纹图谱研究

HPLC Fingerprint of Berchemia polyphylla Wall.ex Lawson

目的建立光枝勾儿茶的高效液相色谱(HPLC)指纹图谱,以评价其药材的质量。方法应用高效液相色谱法,双波长阵列检测器(DAD),乙腈-甲醇-磷酸缓冲液梯度洗脱,测定了10批光枝勾儿茶样品。色谱条件为:Agilent XDBC18(4.6 mm×150 mm,5μm),柱温25℃;流动相A相为乙腈,B相为甲醇,C相为0.05%磷酸梯度洗脱(80%～25%C),分析时间95 min;流速1 ml/min,检测波长280 nm。结果 10批光枝勾儿茶样品得到的高效液相指纹图谱有18个共有峰,经相似度计算,10批药材整体相似性较好。结论光枝勾儿茶药材的指纹图谱特征性及专属性强,可为光枝勾儿茶的质量控制提供依据。

Objective To establish the HPLC fingerprints for identification and evaluation the quality of Berchemia polyphylla Wall.ex Lawson.Methods It is analyzed by HPLC with acetonitrile（A）,methanol（B） and H3PO4（C） as mobile phases in gradient mode.The elute conditions were 0~30min,changed from 80% C to 55% C,20% B to 45% B;30~50 min,55% C to 60% C,to 40% A;50~60 min,60% C to 40% C,40% A to 60% A;65~75 min,40% C to 30% C,60% A to 70% A;80~85 min,30% C to 25% C,70% A to 75% A.The column temperature was set at 25℃ and the flow rate was 1.0 ml·min-1 with the detection wavelength of 254 nm.Results The HPLC chromatographic fingerprint of Berchemia polyphylla Wall.ex Lawson,showing 15 characteristic peaks,was established from 10 lots of Berchemia polyphylla Wall.ex Lawson.After calculating the similarity of fingerprints of 10 patches,the similarity of every patch of Berchemia polyphylla Wall.ex Lawson.was calculated as good in genera.Conclusion The chromatographic fingerprint of Berchemia polyphylla Wall.ex Lawson with high characteristics and specificity can be used to control its quality.