薏苡仁油中甘油三酯含量的快速测定

The Rapid Determination of Triglyceride in Coix Seed Oil

目的建立薏苡仁油中甘油三酯含量的快速测定方法。方法以甘油三油酸酯为对照品,采用紫外-可见光谱法在412 nm处测定薏苡仁油中甘油三酯的含量。结果甘油三油酸酯在0.202~1.010 mg/ml范围内线性关系良好,R2=0.996 6,甘油三酯平均回收率为101.20%,RSD为1.47%。结论该方法简便、快速、结果准确,适用于薏苡仁油中甘油三酯含量的快速测定。

Objective To establish a method for rapid determination of triglyceride content in coix seed oil.Methods The content of triglyceride in Coix seed oil was analyzed by the UV spectro-photometer at 412 nm wavelength with glyceryltrioleate as a standard.Results The standard curve of triglyceride presented the favorable linear correlation over the range of 0.202-1.010 mg/ml with R2=0.996 6.The average recovery rate was 101.20% and RSD was 1.47%.Conclusion The method is simple,quick and accurate,which can be used for the rapid determination of triglyceride in coix seed oil.