不同葛花提取物对乙醇致小鼠肝损伤保护作用的比较研究

Comparison on the Protective Effects of Flos Puerariae lobata Extracts Prepared by Different Solvents on Experimental Alcoholic Liver Disease

目的比较不同提取纯化工艺对葛花提取物中黄酮含量及其保肝作用的影响。方法制备葛花的水提物、乙醇提取物及其大孔树脂纯化物,采用高效液相色谱法分析其中主要黄酮类成分的含量;复制乙醇致小鼠肝损伤模型,同时连续多日灌胃不同葛花提取物,以小鼠体质量变化、肝脏指数、血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和丙二醛(MDA)等指标比较对乙醇所致小鼠肝损伤保护作用的差异。结果葛花不同提取物中黄酮类成分的比例和含量差异明显,其中乙醇提取物黄酮类含量高,其保肝活性更强。结论建议针对不同活性采用不同提取方法制备样品。

Objective To compare the different protective effects of Flos Puerariae lobata（FPL） extracts prepared by different solvents on alcoholic liver disease（ALD） of mice.Methods The 70% ethanol,water extracts of FPL,and their macroporous resin purified products were prepared respectively.The determination of tectoridin and 6＇＇-O-xylosyltectoridin in those extracts was conducted by a high performance liquid chromatography method（HPLC）.The ALD model mice were treated by alcohol i.g.,meanwhile mice in FPL groups and positive control group were treated with different extracts and hepatic protective compound bifendete respectively for 20 days.To evaluate the protective effect,the followings were analyzed at the 20th day： the weight of mice,the liver index,activities of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and the content of liver maleic dialdehyde（MDA）.Results Difference was found between the HPLC determination results of FPL extracts prepared by different solvents and their purified products.All of the extracts had nearly no effect on the weight of ALD mice but could significantly decrease the liver index of model group.AST and ALP in mice from water extract group and purified water extract group were not significantly decreased compared with the ALD model group.The 70% ethanol extract had significant protective effect on ALD,and the purified ethanol extract had even more potent protective effect than that.Conclusion A great difference in the isoflavone content was found in FPL isoflavone extracts prepared by different solvents and methods,whereas various degrees of protective effects were shown on experimental ALD mice accordingly.Ethanol extract had relatively more potent hepatic protective effect on ALD mice.It indicated that different solvents and extraction methods should be applied according to different kinds of pharmacological activity needed in FPL clinical use.