天然冰片小鼠急性毒性的时间毒理学研究

Chronotoxicology Study of Acute Toxicity on Natural Borneol in Mice

目的研究小鼠口服天然冰片后急性毒性的昼夜差异。方法小鼠随机分为白昼组和黑夜组,每组60只,分别于不同时间给予不同剂量的天然冰片,观察死亡情况,计算LD50值。结果 白昼组的LD50为2 425.9 mg/kg,95%可信区间为1939.5～2 940.6 mg/kg;黑夜组的LD50为2 307.3 mg/kg,95%可信区间为1 555.6～2 948.2 mg/kg,白昼组LD50大于黑夜组。结论 天然冰片对小鼠的急性毒性存在昼夜差异,明期(小鼠休息期)毒性小于暗期(小鼠活动期)。

Objective To study the difference of acute toxicity between day and night after oral administration of natural borneol in mice.Methods Mice were randomly divided into day group and night group（n=60）,at different time points with different doses of natural borneol,the mortality was observed and the half lethal dose（LD50） was calculated.Results The LD50 of day group was 2 425.9mg/kg,95% confidence interval was 1 939.5~2 940.6 mg/kg;the LD50 of night group was 2 307.3 mg/kg,95% confidence interval was 1 555.6~2 948.2 mg/kg,and the LD50 of day group was larger than night group.Conclusion The acute toxicity is different between day and night for natural borneol in mice,and the toxicity of day group is less than night group.