一株藏红花内生真菌多糖的提取及抗氧化活性研究

Extraction and Antioxidant Activity of Polysaccharide from the Endophytics Fungus from Crocus Sativus L.

目的首次由藏红花分离得到内生真菌,研究热水浸提法和超声波法提取真菌#CSL-8多糖的最佳工艺及多糖的抗氧化活性。方法通过正交实验探讨真菌多糖提取的最佳工艺,并采用DPPH法对多糖的抗氧化活性进行评价。结果 热水浸提法提取的最佳工艺参数为:提取温度95℃,料液比1∶30,提取时间4 h,粗多糖得率为9.47%,多糖含量为26.35%;超声波法提取的最佳工艺参数为:超声功率400 W,料液比1∶40,提取时间40 min,粗多糖得率为9.12%,多糖含量为57.65%。两种方法提取的多糖对DPPH自由基的`清除率IC50分别为0.15和0.11 mg/m。l结论与热水浸提法相比,超声波法提取得到的多糖含量较高,提取效果较好。抗氧化实验中,两种方法提取的多糖对DPPH自由基均有较强的清除能力,且有明显的量-效相关性。

Objective To study the optimum extraction technology and antioxidant activity of polysaccharides from the fungus #CSL-8,firstly isolated from Crocus Sativus L.Methods The extraction of the polysaccharides was prepared by L9（33） orthogonal test plan,the antioxidant activity of the extract was evaluated by DPPH method,compared with Vc.Results The optimal extraction condition of fungal polysaccharide in water extraction was as follows： extraction temperature 95℃,material-water ratio 1： 30,and extraction time 4h,the rate of extraction was 9.47%,the concentration of polysaccharide in the fungus was 26.35%.The optimal extraction condition of fungal polysaccharide in ultrasonic extraction was as follows： extraction power 400w,material-water ratio 1： 40,and extraction time 40min,the rate of extraction was 9.12%,the concentration of polysaccharide in the fungus was 57.65%.In a certain concentration scope,there was a strong positive correlation between the scavenging activity and the concentrations.The water extraction had an IC50 value of 0.15 mg/ml,and the ultrasonic extraction was 0.11mg/ml.Conclusion The ultrasonic extraction surpassed the water extraction.The tested polysaccharides by two extraction methods has strong ability to scavenge the stable free radical DPPH and shows a concentration-effect relationship.