扶正解毒颗粒对染镍大鼠肾细胞凋亡及Bax、bcl-2表达的影响

Effects of Fuzheng Jiedu Granula on Renal Cell Apoptosis and Expression of Apoptosis Related Proteins in Rats Exposed to Nickel

目的研究中药扶正解毒颗粒(FJG)对硫酸镍(N iSO4)染毒大鼠肾细胞凋亡及凋亡相关蛋白Bax、Bc l-2表达的影响。方法以W istar大鼠N iSO4(2.5 mg.kg-1.d-1)染毒造模,FJG灌胃处理,采用DNA末端标记法(TUNEL)以及免疫组化SP法观察肾细胞凋亡与肾脏Bax和Bc l-2蛋白表达水平。结果 与N iSO4组相比,FJG组大鼠肾脏细胞凋亡率明显减少(P<0.05或P<0.01),Bc l-2表达增强,Bax表达及Bax/Bc l-2比值下调(P<0.05或P<0.01),尤以FJG大、中剂量组作用显著。结论 FJG可能通过降低凋亡相关蛋白Bax和增强Bc l-2的表达而抑制N iSO4诱发的肾细胞凋亡,发挥拮抗镍性肾损伤的作用。

Objective To study the effects of Fuzheng Jiedu Granula（FJG） on renal cell apoptosis and expression of apoptosis related proteins in rats exposed to nickel（NiSO4）.Methods Nephrotoxicity was induced in rats by intraperitoneal injection of NiSO4（2.5 mg·kg-1·d-1） and FJG（5,10,20 mg·kg-1·d-1） was administered by gavage.Apoptosis was evaluated by terminal dexoynucleotide transferase mediatedd-UTP-biotin nick end labeling（TUNEL）.Immunohistochemistry was used to detect the protein expression of Bax and Bcl-2.Results In FJG groups,the apoptosis index（AI） of renal cells was decreased（P0.05,P0.01）,the expression of Bcl-2 was increased and the expression of Bax was decreased significantly（P0.05 or P0.01）,compared with NiSO4 group.Conclusion FJG can suppress renal cell apoptosis induced by NiSO4 and protect renal from injury by increasing the expression of Bcl-2 and reducing the expression of Bax.