咳嗽变异性哮喘从肝论治的实验研究

Experimental Study on Cough Variant Asthma from Liver

目的以敏咳清颗粒、止咳颗粒对体外培养的人胚肺成纤维细胞(lung fibroblast,LFB)产生干细胞因子(stem cellfactor,SCF)与转化生长因子β1(Transforming growth factor-β1,TGF-β1)的影响为依据探讨咳嗽变异性哮喘从肝论治的辨证思路。方法把以药测证的方法应用于实验研究,将常规培养LFB分为空白组、模型组、对照组、敏咳清高浓度组、敏咳清低浓度组、止咳颗粒高浓度组、止咳颗粒低浓度组,加入肝素刺激后分别加入对应的含药血清,采用ELISA法检测各组对LFB表达SCF及TGF-β1的影响。结果 敏咳清颗粒高、低剂量组与止咳颗粒高、低剂量组分别相比较有显著性差异(P<0.05),且敏咳清颗粒可以剂量依赖地抑制LFB产生SCF、TGF-β1。结论 敏咳清颗粒与止咳颗粒相比,能显著降低LFB表达SCF及TGF-β1,揭示了咳嗽变异性哮喘从肝论治的部分分子生物学基础。

Objective To study the effects of Keminqing granule、Zhike granule on expression of stem cell factor（SCF） and transforming growth factor-β1（TGF-β1） in human embryonic lung fibroblast（LFB） and explore the ideas in syndrome differentiation of cough variant asthma from the liver.Methods The human embryonic lung fibroblast was stimulated by liquemine and divided randomly into 7 equal groups：blank group,control group,model group,high dose Keminqing granule group,low dose Keminqing granule group;high dose Zhike granular used group,low dose Zhike granular used group,each group was detected expression of SCF and TGF-β 1 by ELLISA.Results There were significant differences between high,low dose Keminqing granule groups and high,low dose Zhike granule groups and the Keminqing granule presented Dose-dependent inhibition for the expression of SCFand TGF-β1.Conclusion Keminqing granula can significantly reduce the expression of SCF and TGF-β1,which provides some molecular basis for treating cough variant asthma from the liver.