小麦叶绿体DNA提取方法研究

Study on Extraction Method of Wheat Chloroplast DNA

以成熟的中国春小麦叶片为材料,采用改进的高盐-低pH方法提取叶绿体DNA,经琼脂糖凝胶电泳和PCR扩增,结果表明:所提取的小麦叶绿体DNA纯度高(OD260/280=1.89,OD260/230=2.23),适于PCR反应、基因扩增和酶切鉴定等分子操作,为深入研究小麦叶绿体基因组奠定了基础。

An improved high-salt low-pH method was used to extract chloroplast DNA from leaf tissues of wheat cultivar Chinese spring.The agrose gel electrophoresis and PCR amplification showed that wheat chloroplast DNA of high purity（OD260/280=1.89,OD260/230=2.23）wasobtained.The DNA was suitable to be used as template in PCR,gene amplification and enzymedigestion.