樟树种子休眠机制初探

Primary Study on Dormancy Characteristics of Cinnamonum camphora

以樟树种子为试验材料,从种壳透水性和内源抑制物两方面探索种子休眠原因。结果表明:樟树种子坚硬的外壳影响种子的吸水透性;种壳、种仁中的萌发抑制物抑制了白菜种子的萌发率、淀粉酶活性以及白菜幼苗的鲜质量增加。种壳浸提液抑制强度顺序均为:种壳甲醇浸提液>种壳乙醚浸提液>种壳水浸提液,抑制物主要成分为极性物质,白菜种子萌发率依次为8%、52%、80%,淀粉酶活性依次为0.288、0.545、0.686 mg/(g.min),鲜质量依次为8、52、80 g;种仁浸提液抑制强度顺序均为:种仁乙醚浸提液>种仁甲醇浸提液>种仁水浸提液,抑制物主要成分为非极性物质,白菜种子萌发率依次为:2%、10%、76%,淀粉酶活性依次为0.131、0.233、0.672 mg/(g.min),鲜质量依次为2、10、76 g。樟树种子坚硬种壳以及种子中的萌发抑制物是樟树休眠的原因。

This study aimed to investigate the causes of seed dormancy of Cinnamomum camphora from seed testa absorbing water and endogenous inhibitors.The results showed that the lignified seed shell had a negative impact on water permeability.Water,methanol,ether extracts respectively from seed testa and kernel as germination medium,inhibited the germination percentage,amy-lase activity and fresh weight of cabbage.The extracts from seed testa inhibited the germination percen-tage,amylase activity,fresh weight of cabbage as follows：methanol extractether extractwater extract,the inhibitor was a polar substance,the germination percentage was 8%,52% and 80%,the amylase activity was 0.288,0.545 and 0.686 mg/（g·min）,and the fresh weight of cabbage was8 g,52 g,and 80 g.The extracts from seed kernel inhibited the germination percentage,amylase activity and fresh weight of cabbage as follows：ether extractmethanol extractwater extract,the inhibitor was non-polar substance,the germination percentage was 2%,10% and 76%,theamylase activity was 0.131,0.233 and 0.672 mg/（g·min）,and the fresh weight of cabbage was 2 g,10 g and 76 g.The hard seed testa and the inhibitors in the seed were the reasons for the dormancy of Cinnamonum camphora.