开封地区鸡大肠杆菌的分离鉴定与耐药性分析

Identification and Analysis of Drug Resistance of Escherichia coli Isolated from Chicken in Kaifeng Area

为掌握开封地区鸡大肠杆菌的耐药性情况,对来自开封地区疑似鸡大肠杆菌病的12份病料进行病原菌的分离鉴定、形态学观察、生化试验,并对分离株进行了动物接种试验和耐药性分析,结果鉴定出6株鸡大肠杆菌。动物攻毒试验结果显示,6株细菌对雏鸡的发病率均为100%,致死率各不相同。其中KF株对雏鸡的致死率最低,为50%(10/20);LK株对雏鸡的致死率最高,为90%(18/20)。耐药性检测结果表明,分离的6株细菌对链霉素、新霉素、阿莫西林、磺胺二甲嘧啶、环丙沙星、氧氟沙星等均产生了耐药性,并且表现为对5种以上抗生素耐药。敏感药物为头孢噻肟、阿奇霉素、丁胺卡那霉素、氟甲砜霉素等。

In order to investigate the drug tolerance of chicken Escherichia coli（E.coli） in Kaifeng area,identification,morphology observation,biochemical test,animal experiments and the drug tolerance analysis of the isolates from 12 suspected chicken colibacillosis specimens were performed.Finally,a total of 6 isolates were characterized as E.coli and all showed 100% morbi-dity but different fatality to chicken,among which KF strain showed the lowest fatality（50%） while LK strain showed the highest fatality（90%）.These strains had the drug resistance to streptomycin,amoxicillin,sulfadimidine,ciprofloxacin,and ofloxacin.They could resist more than 5 kinds of drugs at the same time.These new strains were susceptible to the drugs such as cefotaxime,azithromycin,amikacin and cextrosulfenidol.