摘要
本实验采用寡聚核苷酸指导的定点突变法,缺失了分别存在于YFD42和YFD58中的a-因子信号肽序列与a-hANP基因和a-因子信号肽序列与a-1FN基因间接头区域的27和18个核苷酸。由于被缺失部分恰好含有一个酶切位点,利用这一特点,酶切检查初步筛选出缺失了一个HindⅢ酶切位点的突变子。经DNA序列分析,证实缺失的核苷酸序列和设计完全一致。
By the method of oligonucleotide-directed mutagenesis, we made 27bp and 18bp deletion at junction region between a-factor signal sequence and α-hANP gene, α-factor signal sequence and α-IFN gene, respectively. Since the deleted region contains one WindⅢ site, the mu-iams without this site were selected. The result of DNA sequence analysis showed that the sequences of the mutants were the same as designed.
