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Lepidium sativum Linn.种子的脱脂乙醇提取物体外自由基清除活性(英文) 被引量:1

In vitro Free Radical Scavenging Potential of Defatted Ethanolic Extract of the Seeds of Lepidium sativum Linn.
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摘要 AIM:The objective of the present study was to evaluate the antioxidant potential of the defatted ethanolic extract of the seeds of Lepidium Sativum Linn.METHODS:Different in vitro chemical assays viz.DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging,ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical scavenging,iron chelation,lipid peroxidation,super-oxide scavenging and non-enzymatic haemoglobin glycosylation assay were used.The total antioxidant capacity of the extract was determined spectrophotometrically by phosphomolybdic acid method.RESULT & CONCLUSION:The defatted lepidium seed extract showed significant free radical scavenging activity in ABTS and non-enzymatic glycosylation assays,and a moderate activity in all the other assays.IC50 of the extract in the DPPH,ABTS,iron chelation,lipid peroxidation and super oxide scavenging assays were found to be 171.13,38.64,128.94,71.39 and 206.09 μg·mL-1 respectively.The haemoglobin glycosylation assay of the extract showed a percentage scavenging of 46.60% and 74.88%,at 0.5 and 1.0 μg·mL-1,concentrations,respectively.Total antioxidant capacity of ethanolic extract of L.sativum (10 mg·mL-1) was found to be equivalent to 58.38 μg·mL-1 of ascorbic acid. AIM:The objective of the present study was to evaluate the antioxidant potential of the defatted ethanolic extract of the seeds of Lepidium Sativum Linn.METHODS:Different in vitro chemical assays viz.DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging,ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical scavenging,iron chelation,lipid peroxidation,super-oxide scavenging and non-enzymatic haemoglobin glycosylation assay were used.The total antioxidant capacity of the extract was determined spectrophotometrically by phosphomolybdic acid method.RESULT CONCLUSION:The defatted lepidium seed extract showed significant free radical scavenging activity in ABTS and non-enzymatic glycosylation assays,and a moderate activity in all the other assays.IC50 of the extract in the DPPH,ABTS,iron chelation,lipid peroxidation and super oxide scavenging assays were found to be 171.13,38.64,128.94,71.39 and 206.09 μg·mL^-1 respectively.The haemoglobin glycosylation assay of the extract showed a percentage scavenging of 46.60% and 74.88%,at 0.5 and 1.0 μg·mL^-1,concentrations,respectively.Total antioxidant capacity of ethanolic extract of L.sativum (10 mg·mL-1) was found to be equivalent to 58.38 μg·mL^-1 of ascorbic acid.
出处 《中国天然药物》 SCIE CAS CSCD 北大核心 2011年第6期435-440,共6页
关键词 DPPH ABTS Lipid peroxidation Nitric oxide Lepidium sativum DPPH ABTS Lipid peroxidation Nitric oxide Lepidium sativum
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  • 1Yu BE Cellular defenses against damage from reactive oxygen species [J]. PhysiolRev, 1994, 74 (1): 139-162.
  • 2Halliwell B, Gutteridge JMC. Free radicals in biology and medicine [M]. 2^nd ed. Oxford: Clarendon Press, 1988.
  • 3Collier A, Small M, Wilson R, et al. Free radical activity in Type 2 diabetes [J]. Diabetic Med, 1990, 7 (1):27-30.[doi: 10.1111/j. 1464-5491.1990. tbO1302.x].
  • 4Baynes JW. Role of oxidative stress in development of complication in diabetes [J]. Diabetes, 1991, 40 (4): 405-412. n: Academic Press, 1995: 495.
  • 5Cotran RS, Kumar V, Collins T. Robbin's pathological basis of diseases [M]. 6th ed. India: Noida, Thomson Press (I) Ltd., 1999: 1.
  • 6Yu BE Suescun EA, Yang SY. Effect of age-related lipid peroxidation on membrane fluidity and phospholipids A2: modulation by dietary restriction [J]. Mech Agieng Dev, 1992, 65 (1): 17-33.
  • 7Campbell IC, Abdulla EM. Strategic approaches to in vitro neurotoxicology, in: Approaches and methods: Neurotoxicology [M]. Londo.
  • 8Halliwell B. Free radicals in biology and medicine [M]. 2^nd ed. New York: Oxford, Clarendon Press, 1989: 236.
  • 9Kerr ME, Bender CM, Monti EJ. An introduction to oxygen free radicals [J]. Heart Lung, 1991, 25 (3): 200-209.
  • 10Halliwell B, Gutteridge JMC. The antioxidants of human extracellular fluids [J]. Arch Biochem Biophys, 1990, 280 (1): 1-8. [ doi: 10.1016/0003-9861 (90)90510-6].

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