丹参酮ⅡA对慢性脑缺血大鼠脑组织的保护机制

Protection mechanisms of tanshinone Ⅱ A to chronic cerebral ischemia in rat brain tissue

目的:探讨丹参酮ⅡA对慢性脑缺血大鼠的保护机制。方法:10～12月龄Wistar大鼠42只,随机分为假手术组、慢性脑缺血组和缺血保护组。假手术组和慢性脑缺血组大鼠在手术次日腹腔注射4ml0.9%NaCl;缺血保护组术后次日腹腔注射丹参酮ⅡA磺酸钠注射液16mg/kg加0.9%NaCl稀释到4ml。4w后,测定三组大鼠认知能力;GFAP免疫组化染色,鉴定阳性细胞数目。结果:缺血保护组大鼠脑组织损伤恢复较好,慢性脑缺血组大鼠脑组织损伤严重恢复差,二者比较有统计学差异(P<0.05)。结论:丹参酮ⅡA通过减轻慢性脑缺血导致的神经损害,达到保护脑组织的作用。

Objective： To discuss the protection mechanisms of tanshinone ⅡA to chronic cerebral ischemia in rat brain tissue. Methods： 10 to 12-month-old Wistar rats were divided into Sham group, ischemic chronic cerebral ischemia group and protection group. Ham group and rats with chronic cerebral ischemia were injectioned of 4ml 0.9% NaC after surgery the next day; ischemic protection group the next day after intraperitoneal injection of sodium tanshinone ⅡA injection, 16mg/kg plus 0.9% NaCl was diluted to 4ml. After 4w, three groups of rats were measured cognitive ability; GFAP immunohistochemistry, identified the number of positive cells.Results： the ischemic brain injury in rats of rotection group recovered well, the ischemic brain injury in rats of ischemic chronic cerebral ischemia group recovered not well, Difference between the two grops was statistically （P 〈0.05）. Conclusion： Tanshinone ⅡA by reducing the chronic nerve damage caused by cerebral ischemia, to protect the brain tissue.