流动相对灰树花多糖分子量测定影响的研究

Mobile Phases Influence on Molecular Weight Determination of Grifola Frondosa Polysaccharides

目的:考察流动相对高效凝胶渗透色谱法(HPGPC)测定灰树花多糖(GFP)相对分子质量(Mr)的影响因素。方法:采用HPGPC法分别以不同的流动相、盐浓度、pH值测定GFP的Mr,并对测定结果进行分析。结果:分别以纯水、0.02%NaN3、0.1mol·L-1NaNO3和0.1mol·L-1Na2SO4溶液为流动相进行测定,GFP的Mr依次为822410、177520、29112和25066道尔顿;在0-0.025mol/L氯化钠溶液中Mr先急剧降低,然后随着盐浓度的增加,在0.1-0.2mol/L氯化钠溶液中趋于平稳;在pH3-6时,Mr随pH增加而急剧增加,在pH6-9范围内变化较小,当pH为10时又稍微增加。结论:不同流动相、盐浓度、pH值对GFP的Mr测定结果有明显影响。

Objective：To investigate the mobile phases influence on relative molecular weight（Mr） determination of Grifola Frondosa Polyaccharide（GFP） by High performance gel permeation chromatography（HPGPC）. Methods： Determine Mr of GFP by HPGPC using different mobile phases, concentrations of NaC1 and pH value of GFP separately. Results：The Mr of GFP are 822410,177520,29112 and 25066 dalton determined by HPGPC using pure water,0. 02%NAN3 solufion,0.1mol·L-1 NaNO3 solution and 0. 1mol·L-1 Na2SO4 solution as mobile phase respectively. The Mr of GFP was sharply dropped under the eoneentration of 0 - 0. 025 mol/L NaCl and then tend to be stable from 0. 1 to 0. 2 mol/L NaC1. The Mr increase quickly from pH 3 to 6 ,and then maintain stable from pH 6 to 9 ,but slightly inereased at pH 10. Conclusion：Different mobile phases, concentrations of NaCl and pH value have great influence on Mr deterroination of GFP.