摘要
本文报道了从被AcNPV感染的大蜡螟中提纯到AcNPV DNA,以质粒pBR325作为载体,从AcNPV基因组DNA EeoRI片段克隆中得到含AcNPV多角体蛋白基因片段的重组质粒。经原位杂交,酶切鉴定,DNA顺序分析,并与Hooft Van Iddekinge等人测定的结果比较,证明筛选到的7.3kb EcoRI片段包含了AcNPV多角体蛋白结构基因及其启动基因。核多角体病毒多角体蛋白启动基因是迄今为止在真核细胞中所发现的最强启动基因之一,是理想的表达外源基因的启动子。
Autographa califonica nuclear polyhedrosis virus (AcNPV) DNA from larvae of lepido-pteran infected by AcNPV has been isolated. The EcoRI fragements of AcNPV DNA have been cloned using plasmid pBR325 as a vector. The polyhedrin gene fragment containing recombin-ant plasmid pAc-18 was isolated through in situ hybrilization. For the convenience of further construction the EcoR1 insertion fragment was cloned into pUC-8. Thus plasmid pUAc-2 was obtained. The results of restriction mapping of pAc-18 and pUAc-2 and part of the sequence were reported. The polyhedrin promotor was known to be one of the most effective promoter useful in the expression of foreign genes in the insect cells.
