摘要
采用实验室内菌饼接种法,以常见的酚酸类化合物对羟基苯甲酸、肉桂酸和甘氨酸、丙氨酸来研究酚酸类物质和氨基酸对西瓜专化型镰刀菌(Fusarium oxysporium f.sp.niveum)生长的影响。结果表明,供试酚酸类物质和氨基酸都对镰刀菌菌丝生长具有一定促进作用。1.00 mmol/L的对羟基苯甲酸和0.10mmol/L的肉桂酸对镰刀菌菌丝促进生长率分别达到9.59%和7.70%,浓度为1.0 g/L的甘氨酸和浓度为0.5 g/L的丙氨酸对镰刀菌菌丝促进生长率分别达到17.29%和13.70%。肉桂酸和丙氨酸溶液浓度增大对菌丝生长的促进作用呈下降趋势,表明低浓度能有效促进镰刀菌菌丝的生长,而高浓度则有利于菌丝色素的产生。试验结果为探究西瓜的自毒物质是否能通过促进西瓜枯萎病致病菌生长的机制来影响连作西瓜病害的发生提供了依据。
The effects of phenolic acids and amino acids on the mycelial growth of Fusarium oxysporium f.sp.niveum were researched by laboratory inoculating mycelial mass.The compounds of phenolic acids and amino acids selected in this study were p-hydroxybenzoic acid,cinnamic acid,glycine and alanine.The results showed that the Fusarium mycelia growth was promoted by phenolic acids and amino acid at some extent.The promoted growth rate could reach 9.59% and 7.70% by 1.00mmol/L of p-hydroxybenzoic acid and 0.10 mmol/L of cinnamic acid respectively.The promoted mycelial growth rate could reach 17.29% and 13.70% by the concentration of 1.0g/L of glycine and the concentration of 0.5g/L of alanine respectively.The promoted role would decline with the increasing of the concentration of cinnamic acid and alanine,which indicated the Fusarium mycelial growth could be obviously promoted by low concentration of cinnamic acid and alanine and the production of mycelial pigment could increase at high concentration.The results provided a basis for the occurrence of disease to explore whether autotoxicity watermelon wilt pathogen through the promotion of mechanisms influenced the growth of watermelon cropping.
出处
《湖北农业科学》
北大核心
2011年第20期4181-4184,共4页
Hubei Agricultural Sciences
基金
国家农业科技华东(江苏)创新中心--农业废弃物资源化工程技术研究中心开放项目(200801)