摘要
利用RT-PCR法对广东烟草样品的马铃薯Y病毒(Potato virus Y,PVY)辅助成分蛋白质(Helpercomponent proteinase,HC-Pro)基因进行了克隆,并对其进行了序列分析。测序结果表明广东PVY HC-Pro(命名为PVY-HC-GD)基因(用PVY-HC-GD表示)全长1 395 bp,编码465个氨基酸。与已报道的PVY HC-Pro基因核苷酸序列相比发现,PVY-HC-GD与PVY NTN株系(PVYNTN)(AB331517)的PVYHC-Pro基因核苷酸序列相似性最高,达99.6%。而氨基酸序列比较表明,PVY-HC-GD与PVY N株系(PVYN)(AM268435)的PVY HC-Pro氨基酸序列相似性最高,为99.1%。进一步将PVY-HC-GD的核苷酸和其编码的氨基酸序列与其他报道的同源基因比对构建了系统关系树,结果显示广东烟草样品中PVY-HC-GD与PVYNTN PVY HC-Pro亲缘关系最近。
Helper component proteinase（HC-Pro） gene was cloned by RT-PCR from tobacco leaves infected with Potato virus Y（PVY） in Guangdong,and then sequenced.The full length of HC-Pro of PVY from tobacco in Guangdong（PVY-HC-GD） was 1 395 bp,encoding 465 amino acids.Sequence analysis showed that PVY-HC-GD shared the highest nucleotide sequence similarity with Potato virus Y strain NTN（AB331517）（99.6%）,and the highest amino acid sequence similarity with Potato virus Y strain N（AM268435）（99.1%）.Phylogenetic trees constructed based on multiple alignment of nucleotide and amino acid sequences of PVY-HC-GD and 17 other PVY indicated that PVY-HC-GD had the closest relationship with Potato virus Y strain NTN.
出处
《湖北农业科学》
北大核心
2011年第20期4298-4301,共4页
Hubei Agricultural Sciences
基金
广东省教育厅优秀青年创新人才培育项目(LYM08065)
