CX37基因I1297D多态性与缺血性脑卒中及临床分型的关系探讨

Study on polymorphisms of connexins37 genes of ischemic stroke and its clinical classification

目的探讨连接蛋白37(connexins37,CX37)基因I1297D多态性位点与缺血性脑卒中及其亚型的关系。方法采用限制性片段长度多态性分析技术,检测缺血性脑卒中组232例(根据诊断分为动脉粥样硬化性血栓性脑梗死组115例,脑栓塞组31例,腔隙性脑梗死组86例)和健康对照组235例CX37基因I1297D多态的分布。结果 CX37基因I1297D多态性,缺血性脑卒中组和对照组中均以II基因型和I等位基因为主。基因型(II型I、D型、DD型)I、和D等位基因分布频率在缺血性脑卒中组分别为46.56%、39.22%、14.22%、66.16%和33.84%,对照组分别为47.66%、43.40%、8.94%、69.36%和30.64%,2组比较差异无统计学意义(P>0.05),且其基因型、等位基因分布频率在缺血性脑卒中各亚型之间与对照组比较,差异无统计学意义(P>0.05)。结论 CX37基因I1297D的多态性与缺血性脑卒中易感性无关。

Objective To investigate the association between I1297D polymorphism of connexins37 gene and ischemic stroke.Methods Two hundred and thirty-two patients with ischemic stroke（115 cases of atherosclerotic thrombotic cerebral infarction,86 cases of lacunar infarction and 31 cases of cerebral embolism）and 235 healthy controls were recruited.Polymerase chain reaction followed by restriction fragment length polymorphism（PCR-RFLP）was used to detect I1297D polymorphism of connexins37 gene.Results The results showed that CX37 II genotype and I allele in the two groups were dominant genotypes respectively.The frequencies of II,ID,DD genotype and I,D allele were 46.56%,39.22%,14.22%,66.16%,33.84% in patients with ischemic stroke,and 7.66%,43.40%,8.94%,69.36%,30.64% respectively in the controls,no difference was found between ischemic stroke and healthy subjects（P0.05）,no difference was found between all of subtypes of ischemic stroke and healthy subjects（P0.05）,too.Conclusion CX37gene I1297D polymorphism is not associated with an increased risk of ischemic stroke.