炼苗过程中不同光照强度对白及内源激素的影响

Effects of light intensities endogenous hormones of Bletilla striata during acclimatization

目的:研究不同光照强度对白及无菌苗炼苗移栽过程中内源激素的影响,阐明光照影响白及炼苗成活的可能机制,为白及资源保护和栽培提供理论依据。方法:设置3种光照强度(3 000,6 000,12 000 lx)进行炼苗移栽实验,研究光照强度对内源激素IAA,ABA,GA3含量的影响。结果:移栽炼苗期内源IAA含量明显下降,以中光照强度处理下降最多;内源ABA在短期内迅速升高,以中光照强度处理升高最快,第10天时为320.8 ng.g-1比无菌苗高380.2%;内源GA3含量明显下降,以高光照强度处理升高最快,第10天时为473.6 ng.g-1,比无菌苗低52.2%。结论:中等光照强度能够在短时间内迅速改变内源激素含量,尤其是显著提高ABA的含量。

Objective： The effect of light intensities on the endogenous hormones of Bletilla striata during seedling acclimatization was studied in order to explain the mechanism and provide basic data for conservation and cultivation of this wild medicinal species. Method： The samples was treated with three photosynthetic photon flux densities ： 3 000,6 000,12 000 lx during the acclimatization stage to study the hormone dynamic changes of IAA, ABA, GA3. Result： Endogenous IAA concentration was declined in all treatments and the lowest level in all treatments was the middle light intensity. A sharp increase of endogenous ABA concentration in a short period, the concentration was increased by 380.2% in middle light （320.8 ng · g^-1 ）. Endogenous GA3 concentration was declined especially in the high light treatment. At 10 days of acclimatization the GA3 concentration was decreased by 52.2% in the high light （473.6 ng· g^-1 ）. Result ： Endogenous hormones concentration could be rapidly changed by the light, and endogenous ABA concentration could be sharply increased when acclimatization in middle light intensities.