摘要
目的:研究枳壳提取物的工艺。方法:以柚皮苷为指标,考察了提取条件、树脂类型选择,并优化了径高比、上样量、上样流速,洗脱溶媒的浓度、用量及速度,树脂重复使用次数等。结果:提取条件pH 11的NaOH溶液作为提取溶媒,提取3次,每次1 h;选择D101型大孔吸附树脂,上样量为树脂质量-生药1∶0.8,上样流速2 BV.h-1,4倍柱体积的50%乙醇洗脱。结论:本工艺所得提纯物中柚皮苷含量能达到30%以上,简单易行。
Objective： To optimize the method of Fructus Auranti extracts preparation. Method： The extraction conditions and resin type were examined by using naringin as main indices. The sampling amount, the elution solvent and their flow rates were optimized. The recycling times and recovery capacity of resin were also studied. Result： The best extraction could be obtained by adding 10 times amount of NaOH （pH 11 ） for 3 times, 1 hour each time. The purification conditions were specified as follows： using D101 macroporous resin, the sampling ratio of resin weight to raw material was 1： 0. 8 with a flow rate of 2 BV·h^-1 and 4 BV 50% aqueous ethanol as elusion solven. Conclusion： By using this method, the naringin in the product could reach above 30%. Besides, the optimum method is simple and practical.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第21期2971-2974,共4页
China Journal of Chinese Materia Medica
关键词
枳壳
大孔树脂
柚皮苷
Fructus Auranti
macroporous resin
naringin
