羊齿天门冬根中酚酸类化学成分研究

Phenolic compounds from roots of Asparagus filicinus

目的研究羊齿天门冬Asparagus filicinus根的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱及制备薄层色谱等技术进行分离纯化,通过波谱分析(MS、1H-NMR、13C-NMR)鉴定化合物结构。结果从羊齿天门冬根90%乙醇提取物的醋酸乙酯部分分离得到16个酚酸类化合物,分别鉴定为3′-methoxynyasin(1)、(+)-nyasol(2)、()-4′-O-methyl-nyasol(3)、iso-agatharesinol(4)、gobicusin A(5)、4-[5-(4-hydroxyphenoxy)-3-penten-1-ynyl]phenol(6)、1-methoxy-2-hydroxyl-4-[5-(4-hydroxy phenoxy)-3-penten-1-ynyl]phenol(7)、gobicusin B(8)、1-O-p-coumaroyl-3-O-feruloyl-glycerol(9)、1,3-di-O-feruloylglycerol(10)、丁香酸(11)、对羟基苯甲酸(12)、阿魏酸(13)、咖啡酸(14)、反式松柏醇(15)、香草酸(16)。结论除化合物2和12外,其他化合物均为首次从该植物中分离得到。

Objective To study the chemical constituents from the roots of Asparagus filicinus. Methods Silica gel, Sephadex LH-20, ODS column chromatography, and preparative TLC were used to isolate and purify the compounds. All the compounds were identified based on spectral analysis （including MS, ^1H-NMR, and ^13C-NMR）. Results Sixteen phenolic compounds were isolated from 90% ethanol extract in the roots of A. filicinus and were characterized as 3＇-methoxynyasin （1）, （＋）-nyasol （2）, （-）-4＇-0- methyl-nyasol （3）, iso-agatharesinol （4）, gobicusin A （5）, 4-[5-（4-hydroxyphenoxy）-3-penten-l-ynyl] phenol （6）, 1-methoxy-2- hydroxy-4-[5-（4-hydroxyphenoxy）-3-penten-l-ynyl] phenol （7）, gohicusin B （8）, 1-O-p-coumaroyl-3-O-feruloylglycerol （9）, 1, 3-di-O-feruloylglycerol （10）, syringic acid （11）, 4-hydroxybenzoic acid （12）, fumalic acid （13）, caffeic acid （14）, trans-coniferyl alcohol （15）, and vanillic acid （16）, respectively. Conclusion These phenolic compounds are isolated from A. filicinus for the first time except compounds 2 and 12.