用染色体原位杂交技术定位RFLP探针Fr.3-42于16p13

Localization of RFLP Probe Fr. 3-42 at 16p 13 Band by in situ Hybridization

限制性片段长度多态性(RFLP)探针Fr.3-42(第九届人类基因定位国际会议编号D1(?)S21)为一长1.9kb的人类单拷贝EcoRI/HindⅢ片段,本实验采用染色体原位杂交方法,将该探针定位于16号染色体短臂末端(p13)。在另一研究中已证实Fr.3-42与人α-珠蛋白基因紧密连锁。

Recombinant plasmid clone Fr. 3-42(HGM9 no. D16S21) containing a human single-copy DNA segment of 1.9kb was hybridized to human chromosome preparations. Twenty-eight percent of hybridized metaphases exhibited silver grains at the terminal region of short arm of chromosome 16 (16pl3). Close linkage between this probe and the human alpha 1 hemoglobin gene was demonstrated in another study.